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Abstract

A group of five bacilli, designated strains 4T12, 4T19, 5M45, 5M53 and 5T52, isolated from cotton-waste composts for mushroom cultivation, were examined. These strains were Gram-positive, aerobic, motile, spore-forming rods. 16S rRNA gene sequence analyses revealed that the isolates belonged to the genus , showing the highest levels of similarity (approx. 96.6–96.9 %) with respect to DSM 16534. The values for DNA–DNA hybridization (approx. 85–96 %) among these five strains revealed that they belong to the same species. The major menaquinone present was MK-7 and the predominant cellular fatty acids were anteiso-C (approx. 24.5–33.9 %) and C (approx. 15.1–34.1 %). The DNA G+C contents were 37.7–40.9 mol%. On the basis of physiological, biochemical, chemotaxonomic and comparative genomic analyses, the five isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 4T19 (=KACC 11279 =DSM 17723).

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2007-08-01
2019-10-17
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Supplements

vol. , part 8, pp. 1909 - 1913

Phase-contrast micrographs of isolates 4T12, 4T19 , 5M45, 5M53 and 5T52.

Phenotypic characteristics of the five novel isolates.

DNA–DNA hybridization between the five novel isolates.

Fatty acid compositions of the five novel isolates.

[PDF file of Supplementary Fig. S1 and Supplementary Tables S1–S3](68 KB)



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