1887

Abstract

A Gram-negative, rod-shaped, non-spore-forming bacterium (strain AR107) was isolated from the drinking water distribution system of Seville (Spain). A polyphasic taxonomic study of the isolate resulted in its identification as a member of the genus . On the basis of 16S rRNA gene sequence comparisons, strain AR107 was shown to belong to the phylum , being related to members of the genus . It showed 95.2 % sequence similarity with respect to the type strains of and , and 94.1 % similarity with respect to the type strain of . The predominant fatty acids were iso-C, iso-C 3-OH and summed feature 3 (iso-C 2-OH and/or C 7), which supports the affiliation of strain AR107 to the genus . The DNA G+C content of this strain was 38 mol%. On the basis of the phenotypic, phylogenetic and genotypic results, strain AR107 represents a novel species, for which the name sp. nov. is proposed. The type strain is AR107 (=CCM 7347=CECT 7114=JCM 13454).

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2006-08-01
2024-12-02
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References

  1. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  3. Christensen W. B. 1946; Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. J Bacteriol 52:461–466
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1974 Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press;
    [Google Scholar]
  5. Felsenstein J. 1991; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
    [Google Scholar]
  6. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  7. Kersters K., Hinz K.-H., Hertle A., Segers P., Lievens A., Siegmann O., De Ley J. 1984; Bordetella avium sp. nov., isolated from the respiratory tracts of turkeys and other birds. Int J Syst Bacteriol 34:56–70 [CrossRef]
    [Google Scholar]
  8. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  9. Kovács N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703–704
    [Google Scholar]
  10. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  11. Margesin R., Spröer C., Schumann P., Schinner F. 2003; Pedobacter cryoconitis sp. nov., a facultative psychrophile from alpine glacier cryoconite. Int J Syst Evol Microbiol 53:1291–1296 [CrossRef]
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  14. Mellado E., Moore E. R. B., Nieto J. J., Ventosa A. 1995; Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui , Volcaniella eurihalina and Deleya salina , and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45:712–716 [CrossRef]
    [Google Scholar]
  15. Miller L. T. 1982; A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  16. Owen R. J., Hill L. R. 1979; The estimation and base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists , 2nd edn. pp  217–296 Edited by Skinner F. A., Lovelock D. W. London: Academic Press;
    [Google Scholar]
  17. Reasoner D. J., Geldreich E. E. 1985; A new medium for enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Shivaji S., Chaturvedi P., Reddy G. S. N., Suresh K. 2005; Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 55:1083–1088 [CrossRef]
    [Google Scholar]
  20. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria , 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  21. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  22. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. 1998; Classification of heparinolytic bacteria into a new genus, Pedobacter , comprising four species: Pedobacter heparinus comb.nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov.Proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48:165–177 [CrossRef]
    [Google Scholar]
  23. Takeuchi M., Yokota A. 1992; Proposals of Sphingobacterium faecium sp. nov., Sphingobacterium piscium sp. nov., Sphingobacterium heparinum comb. nov. and two genospecies of the genus Sphingobacterium , and synonymy of Flavobacterium yabuuchiae and Sphingobacterium spiritivorum . J Gen Appl Microbiol 38:465–482 [CrossRef]
    [Google Scholar]
  24. Vanparys B., Heylen K., Lebbe L., De Vos P. 2005; Pedobacter caeni sp. nov., a novel species isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55:1315–1318 [CrossRef]
    [Google Scholar]
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