1887

Abstract

Two psychrophilic flavobacteria, designated AT1042 and AT1048, were isolated from terrestrial samples from the Antarctic. Results of 16S rRNA gene sequence analyses indicated a close relationship of these isolates to (96.9 % similarity for strain AT1042) and (97.0 % for strain AT1048). Cells were non-motile and non-gliding. Flexirubin-type pigments were absent. Both isolates were psychrophilic, with an optimum and maximum growth temperature of about 15 and 20 °C, respectively. The major isoprenoid quinone, predominant cellular fatty acids and DNA G+C contents (35–37 mol%) were consistent with the placement of the Antarctic isolates in the genus . Phylogeny based on 16S rRNA gene sequences and several phenotypic characteristics could be used to differentiate these isolates from recognized species. Despite high 16S rRNA gene sequence similarity (98.9 %) between strains AT1042 and AT1048, they represented two distinct species as demonstrated by low genomic relatedness (34 %) and a number of differential phenotypic characters. The polyphasic data presented in this study indicated that the new isolates should be classified within two novel species in the genus . The names sp. nov. (type strain AT1042=IMSNU 14048=KCTC 12223=JCM 12384) and sp. nov. (type strain AT1048=IMSNU 14050=KCTC 12224=JCM 12385) are proposed for these Antarctic isolates.

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2006-06-01
2019-12-13
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References

  1. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  2. Bernardet, J. F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  3. Christensen, W. B. ( 1946; ). Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. J Bacteriol 52, 461–466.
    [Google Scholar]
  4. Christensen, W. B. ( 1949; ). Hydrogen sulfide production and citrate utilization in the differentiation of enteric pathogens and coliform bacteria. Research Bulletin no. 1. Greeley, CO: Weld County Health Department.
  5. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  6. Chun, J., Seong, C. N., Bae, K. S., Lee, K. J., Kang, S. O., Goodfellow, M. & Hah, Y. C. ( 1998; ). Nocardia flavorosea sp. nov. Int J Syst Bacteriol 48, 901–905.[CrossRef]
    [Google Scholar]
  7. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  8. Collins, M. D. ( 1985; ). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366.
    [Google Scholar]
  9. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1993; ). phylip – Phylogeny Inference Package, version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  12. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  13. Isaksen, M. F. & Jørgensen, B. B. ( 1996; ). Adaptation of psychrophilic and psychrotrophic sulfate-reducing bacteria to permanently cold marine environments. Appl Environ Microbiol 62, 408–414.
    [Google Scholar]
  14. Jeon, Y.-S., Chung, H., Park, S., Hur, I., Lee, J.-H. & Chun, J. ( 2005; ). jphydit: a java-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21, 3171–3173.[CrossRef]
    [Google Scholar]
  15. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  16. Kim, B. S., Oh, H. M., Kang, H. & Chun, J. ( 2005; ). Archaeal diversity in tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol 43, 144–151.
    [Google Scholar]
  17. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  19. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athayle, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  20. Ratkowsky, D. A., Lowry, R. K., McMeekin, T. A., Stokes, A. N. & Chandler, R. E. ( 1983; ). Model for bacterial culture growth rate throughout the entire biokinetic temperature range. J Bacteriol 154, 1222–1226.
    [Google Scholar]
  21. Reichenbach, H. ( 1992; ). The order Cytophagales. In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  22. R Foundation for Statistical Computing ( 2003; ). R version 1.8.1. Vienna: Vienna University of Technology. http://www.r-project.org
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Washington, DC: American Society for Microbiology.
  25. Swofford, D. L. ( 1998; ). paup – Phylogenetic Analysis Using Parsimony, version 4. Sunderland, MA: Sinauer Associates.
  26. Tamaki, H., Hanada, S., Kamagata, Y., Nakamura, K., Nomura, N., Nakano, K. & Matsumura, M. ( 2003; ). Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int J Syst Evol Microbiol 53, 519–526.[CrossRef]
    [Google Scholar]
  27. Thornley, M. J. ( 1960; ). The differentiation of Pseudomonas from other gram-negative bacteria on the basis of arginine metabolism. J Appl Bacteriol 23, 37–52.[CrossRef]
    [Google Scholar]
  28. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2005; ). Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55, 769–772.[CrossRef]
    [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  30. Weeks, O. B. ( 1981; ). Preliminary studies of the pigments of Flavobacterium breve NCTC 11099 and Flavobacterium odoratum NCTC 11036. In The Flavobacterium–Cytophaga Group, pp. 108–114. Edited by O. B. Weeks. Weinheim: Gesellschaft für Biotechnologische Forschung.
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