1887

Abstract

Two denitrifying strains of heterotrophic, facultatively anaerobic bacteria, designated ZD1 and ZT1, were isolated from sediment samples collected from mangrove ecosystems in Taiwan. The isolates were Gram-negative. Cells grown in broth cultures were straight rods that were motile by means of a single polar flagellum. The isolates grew optimally in 1–3 % NaCl, but NaCl was not an absolute requirement for growth; only strain ZT1 grew in 13–14 % NaCl. Both isolates grew between 10 and 45 °C, with optimum growth at 30–35 °C. They were capable of anaerobic growth by denitrifying metabolism using nitrate or nitrous oxide as terminal electron acceptors or, alternatively, by fermenting glucose, sucrose or mannitol as substrates. C 7 was the most abundant fatty acid (32.6–35.7 %). The other major fatty acids included C 7 (27.5–29.4 %) and C (20.1–22.0 %). The two isolates had 16S rRNA gene sequence similarity of 96.8 % and shared 94.1–96.8 % sequence similarity with the most closely related species, , , and . They could be distinguished from these species in that they were capable of fermentative metabolism, had relatively high DNA G+C contents (62.0–64.0 mol%) and contained C 7 instead of C 7 as the most abundant fatty acid. Characterization data accumulated in this study revealed that the two denitrifying isolates could be classified as representatives of two novel species in a new genus, gen. nov., with sp. nov. (type strain ZD1=BCRC 17493=JCM 13380) as the type species and sp. nov. (type strain ZT1=BCRC 17494=JCM 13381) as a second species.

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2006-06-01
2019-10-15
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Electron micrographs of strains ZD1 (a) and ZT1 (b) grown in PY broth, showing a single flagellum at one pole of the rod-shaped cells. Bars, 1 µm.

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Electron micrographs of strains ZD1 (a) and ZT1 (b) grown in PY broth, showing a single flagellum at one pole of the rod-shaped cells. Bars, 1 µm.

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