1887

Abstract

A Gram-negative, rod-shaped, -like bacterial strain, DS-4, was isolated from soil of Dokdo, Korea, and its taxonomic position was investigated using a polyphasic approach. Strain DS-4 grew optimally on trypticase soy agar medium without NaCl at pH 6.0–6.5 and 25 °C. It contained Q-10 as the predominant ubiquinone and C 7, C, C 2-OH and C 7 and/or iso-C 2-OH as the major fatty acids. Sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and unidentified phospholipid were the major polar lipids. The DNA G+C content was 66.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-4 fell within the evolutionary radiation comprising species. Levels of 16S rRNA gene sequence similarity between strain DS-4 and the type strains of species ranged from 93.0 to 97.6 %. DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain DS-4 differs from the recognized species. On the basis of phenotypic, phylogenetic and genetic data, this strain represents a novel species of the genus , for which the name sp. nov. is proposed, with DS-4 (=KCTC 12541=CIP 108841) as the type strain.

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2006-09-01
2019-10-20
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