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Abstract

A Gram-positive, non-spore-forming isolate, designated YIM 70212, was isolated from a hypersaline soil sample collected from Qinghai, north-west China. Cells of the isolate were orange-pigmented, motile cocci with multiple flagella. A polyphasic taxonomic investigation was carried out on the isolate. The organism grew at 10–45 °C and pH 7.5–11.0, with optimum growth at 28 °C and pH 8.0–9.5. Strain YIM 70212 grew optimally in the presence of 10 % NaCl, KCl or MgCl.6HO and growth was observed in 1–25 % NaCl, KCl or MgCl.6HO. The peptidoglycan type was A1. Ribose and minor amounts of galactose were detected as the whole-cell sugars. MK-5 was the only menaquinone. The major cellular fatty acids were ai-C (52.4 %) and ai-C (26.5 %). The DNA G+C content was 47.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 70212 formed a distinct lineage within the order and was most closely related to members of the genus , showing 16S rRNA gene sequence similarity levels of 91.0–91.4 %. Based on the high 16S rRNA gene sequence divergence and differences in phenotypic characteristics, it is proposed that the unknown strain be classified in a novel genus and species with the name gen. nov., sp. nov.; the type strain of is YIM 70212 (=KCTC 3943=DSM 17008).

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2006-06-01
2020-01-21
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Comparison of cellular fatty acid compositions of strain YIM 70212 , DSM 20408 and YIM 70157 . [PDF](44 KB)

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Transmission electron micrographs of cells of strain YIM 70212 after 24 h growth on ISP2 agar medium containing 10 % KCl (w/v) at 28 °C. Bars, 1 µm.

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Transmission electron micrographs of cells of strain YIM 70212 after 24 h growth on ISP2 agar medium containing 10 % KCl (w/v) at 28 °C. Bars, 1 µm.

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