1887

Abstract

A thermotolerant, Gram-positive, aerobic, endospore-forming, acidophilic bacterium (strain Kr1) was isolated from the pulp of a gold-containing sulfide concentrate processed at 40 °C in a gold-recovery plant (Siberia). Cells of strain Kr1 were straight to slightly curved rods, 0.8–1.2 μm in diameter and 1.5–4.5 μm in length. Strain Kr1 formed spherical and oval, refractile, subterminally located endospores. The temperature range for growth was 20–60 °C, with an optimum at 40 °C. The pH range for growth on medium containing ferrous iron was 1.2–2.4, with an optimum at pH 2.0; the pH range for growth on medium containing S was 2.0–5.0, with an optimum at pH 2.5. Strain Kr1 was mixotrophic, oxidizing ferrous iron, S, tetrathionate or sulfide minerals as energy sources in the presence of 0.02 % yeast extract or other organic substrates. The G+C content of the DNA of strain Kr1 was 48.2±0.5 mol%. Strain Kr1 showed a low level of DNA–DNA reassociation with the known species (11–44 %). 16S rRNA gene sequence analysis revealed that Kr1 formed a separate phylogenetic group with a high degree of similarity between the nucleotide sequences (98.3–99.6 %) and 100 % bootstrap support within the phylogenetic cluster. On the basis of its physiological properties and the results of phylogenetic analyses, strain Kr1 can be affiliated to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Kr1 (=VKM B-2339=DSM 17362).

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2006-05-01
2020-01-25
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