1887

Abstract

Two moderately halotolerant Gram-negative bacteria were isolated from tidal flat sediment of the South Sea in Korea (the Korea Strait). The strains, designated M9 and M18, were strictly aerobic, rod-shaped and non-spore-forming and motile with a flagellum and their major fatty acids were C and C cyclo 8. Strains M9 and M18 could grow in the presence of up to 13–15 % (w/v) NaCl, but their optimum salt concentrations were relatively low (0–3 %, w/v). The major predominant isoprenoid quinone was Q-8 and the G+C content of the genomic DNA was 57–58 mol%. Phylogenetic analyses and comparative 16S rRNA gene sequence studies revealed that strains M9 and M18 formed a phylogenetic lineage distinct from the genus within the class and were most closely related to the genera , and , with less than 92·5 % 16S rRNA gene sequence similarity. The level of 16S rRNA gene sequence similarity between the two strains was 96·7 %. On the basis of physiological and phylogenetic properties, strains M9 and M18 represent separate species within a novel genus of the class , for which the names gen. nov., sp. nov. (type species) and sp. nov. are proposed. The type strains of and are M9 (=KCTC 12356=DSM 16974) and M18 (=KCTC 12357=DSM 16975), respectively.

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2006-03-01
2019-10-21
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Transmission electron micrograph showing general morphology of a negatively stained cell of strain M18 from an exponentially growing culture. Bar, 1 µm.

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Colonies of strain M18 showing agarolytic activity.

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