1887

Abstract

Five novel strains isolated from hypersaline soils in China were subjected to a polyphasic analysis to determine their taxonomic position. All of the novel isolates could grow on agar plates at NaCl concentrations of up to 18 % (w/v), with optimum growth at 5–8 %. The DNA G+C contents of the novel strains ranged from 67.9 to 73.2 mol%. The morphological and chemotaxonomic characteristics of the isolates matched those described for members of the genus . Based on their 16S rRNA gene sequence analysis, DNA–DNA hybridization values and phenotypic characteristics, including the composition of cell-wall amino acids and sugars, menaquinones, polar lipids and cellular fatty acids, the isolates are proposed as representing five novel species of the genus . The novel species are proposed as sp. nov. [type strain YIM 90087 (=KCTC 19006=CCTCC AA 2040012=DSM 44841)], sp. nov. [type strain YIM 90094 (=KCTC 19007=CCTCC AA 2040013=DSM 44842), sp. nov. [type strain YIM 90096 (=KCTC 19049=CCTCC AA 2040014=DSM 44843), sp. nov. [type strain YIM 90109 (=KCTC 19008=CCTCC AA 2040015=DSM 44844) and sp. nov. [type strain YIM 90130 (=KCTC 19009=CCTCC AA 2040016=DSM 44845). On the basis of the chemotaxonomic data, the description of the recently described species Li . 2004 is emended.

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2006-05-01
2019-10-18
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References

  1. Al-Tai, A. M. & Ruan, J.-S. ( 1994; ). Nocardiopsis halophila sp. nov., a new halophilic actinomycete isolated from soil. Int J Syst Bacteriol 44, 474–478.[CrossRef]
    [Google Scholar]
  2. Al-Zarban, S. S., Abbas, I., Al-Musallam, A. A., Steiner, U., Stackebrandt, E. & Kroppenstedt, R. M. ( 2002; ). Nocardiopsis halotolerans sp. nov., isolated from salt marsh soil in Kuwait. Int J Syst Evol Microbiol 52, 525–529.
    [Google Scholar]
  3. Chun, J., Bae, K.-S., Moon, E.-Y., Jung, S.-O., Lee, H.-K. & Kim, S.-J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  4. Cui, X. L., Mao, P. H., Zeng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L. ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
    [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  9. Hozzein, W. N., Li, W.-J., Ali, M. I. A., Hammouda, O., Mousa, A. S., Xu, L.-H. & Jiang, C.-L. ( 2004; ). Nocardiopsis alkaliphila sp. nov., a novel alkaliphilic actinomycete isolated from desert soil in Egypt. Int J Syst Evol Microbiol 54, 247–252.[CrossRef]
    [Google Scholar]
  10. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  11. Jahnke, K.-D. ( 1992; ). basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef]
    [Google Scholar]
  12. Kämpfer, P., Busse, H.-J. & Rainey, F. A. ( 2002; ). Nocardiopsis compostus sp. nov., from the atmosphere of a composting facility. Int J Syst Evol Microbiol 52, 621–627.
    [Google Scholar]
  13. Kelly, K. L. ( 1964; ). Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  14. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  15. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  16. Kroppenstedt, R. M. ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationary phases. J Liquid Chromatogr 5, 2359–2387.[CrossRef]
    [Google Scholar]
  17. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  18. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  19. Li, M.-G., Li, W.-J., Xu, P., Cui, X.-L., Xu, L.-H. & Jiang, C.-L. ( 2003; ). Nocardiopsis xinjiangensis sp. nov., a halophilic actinomycete isolated from saline soil sample in China. Int J Syst Evol Microbiol 53, 317–321.[CrossRef]
    [Google Scholar]
  20. Li, W.-J., Park, D.-J., Tang, S.-K., Wang, D., Lee, J.-C., Xu, L.-H., Kim, C.-J. & Jiang, C.-L. ( 2004; ). Nocardiopsis salina sp. nov., a novel halophilic actinomycete isolated from a saline soil in China. Int J Syst Evol Microbiol 54, 1805–1809.[CrossRef]
    [Google Scholar]
  21. Locci, R. ( 1989; ). Streptomycetes and related genera. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2451–2508. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  22. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  23. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  24. Meyer, J. ( 1976; ). Nocardiopsis, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 487–493.[CrossRef]
    [Google Scholar]
  25. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of isoprenoid quinines and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  26. Sabry, S. A., Ghanem, N. B., Abu-Ella, G. A., Schumann, P., Stackebrandt, E. & Kroppenstedt, R. M. ( 2004; ). Nocardiopsis aegyptia sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 54, 453–456.[CrossRef]
    [Google Scholar]
  27. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  28. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16.
    [Google Scholar]
  29. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  30. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  31. Tang, S.-K., Li, W.-J., Wang, D., Zhang, Y.-G., Xu, L.-H. & Jiang, C.-L. ( 2003; ). Studies of the biological characteristics of some halophilic and halotolerant actinomycetes isolated from saline and alkaline soils. Actinomycetologica 17, 6–10.[CrossRef]
    [Google Scholar]
  32. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  33. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  34. Xu, P., Li, W.-J., Xu, L.-H. & Jiang, C.-L. ( 2003; ). A microwave based method for genomic DNA extraction from Actinomycetes. Microbiology (Beijing) 30, 82–84 (in Chinese).
    [Google Scholar]
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