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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 56, Issue 6

sp. nov., a group of diazotrophs isolated from tropical molasses grass Free

Abstract

Fifteen bacterial strains isolated from molasses grass ( Beauv.) were identified as nitrogen-fixers by using the acetylene-reduction assay and PCR amplification of gene fragments. These strains were classified as a unique group by insertion sequence-PCR fingerprinting, SDS-PAGE protein patterns, DNA–DNA hybridization, 16S rRNA gene sequencing and morphological characterization. Phylogenetic analysis of the 16S rRNA gene indicated that these diazotrophic strains belonged to the genus and were closely related to (with 97.5 % similarity). In all the analyses, including in addition phenotypic characterization using Biolog MicroPlates and comparison of cellular fatty acids, this novel group was found to be different from the most closely related species, . Based on these data, a novel species, sp. nov., is proposed for these endophytic diazotrophs of , with TMCY 0552 (=CCBAU 5106001=LMG 23364=CGMCC 1.5340) as the type strain.

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Keyword(s): ARA, acetylene-reduction assay and IS-PCR, insertion sequence-PCR
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2006-06-01
2025-01-18
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Azospirillum melinis sp. nov., a group of diazotrophs isolated from tropical molasses grass
Int J Syst Evol Microbiol 56, 1263 (2006); https://doi.org/10.1099/ijs.0.64025-0
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