1887

Abstract

A polyphasic taxonomic study was carried out to determine the taxonomic position of a newly isolated denitrifying bacterium, designated Slu-05, which had been isolated from sludge from the main aerobic treatment tanks of a municipal sewage treatment plant. Phylogenetic analysis based on comparative 16S rRNA gene sequencing indicated that strain Slu-05 was closely related to LMG 19178 (96.4 % sequence similarity), the sole species in the genus . Strain Slu-05 comprised Gram-negative, motile, non-spore-forming and slightly curved rods. The predominant respiratory lipoquinone was Q-8. The major fatty acids were C 7, C, C isomers and C 3-OH. The G+C content of the genomic DNA was 65.6 mol%. The results of DNA–DNA hybridization (15.6 %) together with phenotypic determination showed that strain Slu-5 could be distinguished from . Moreover, some phenotypic properties concerning enzyme activity, the substrates utilized as carbon sources and growth conditions distinguish strain Slu-5 from . On the basis of the results obtained in this study, Slu-05 (=DSM 17719=KCTC 12530=CCBAU 10199) is the type strain of a novel species of , for which the name sp. nov. is proposed.

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2006-05-01
2020-01-20
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