Gram-positive, rod- or coccoid-shaped bacterial strains KSL-17, KSL-18 and KSL-19 were isolated from an alkaline soil in Korea. They were subjected to analysis using polyphasic taxonomy. Strains KSL-17, KSL-18 and KSL-19 grew optimally at pH 8·0 and 28 °C and in the presence of 0·5 % (w/v) NaCl. They were characterized chemotaxonomically as having -2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H) as the predominant menaquinone and iso-C as the major fatty acid. Their DNA G+C contents were in the range 74·6–74·8 mol%. Strains KSL-17, KSL-18 and KSL-19 were identical in terms of their 16S rRNA gene sequences and exhibited a mean level of DNA–DNA relatedness of 85–90 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains KSL-17, KSL-18 and KSL-19 formed a distinct phylogenetic lineage within the genus . The levels of 16S rRNA gene sequence similarity between the three strains and the type strains of species were in the range 92·6–95·2 %. On the basis of phenotypic, genetic and phylogenetic data, strains KSL-17 (=KCTC 19039=DSM 16315), KSL-18 and KSL-19 should be classified as members of a novel species of the genus , for which the name sp. nov. is proposed.


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vol. , part 1, pp. 271-275

Cellular fatty acid content of strains KSL-17 , KSL-18 and KSL-19. [PDF](22 KB)

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