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Abstract

Gram-positive, rod- or coccoid-shaped bacterial strains KSL-17, KSL-18 and KSL-19 were isolated from an alkaline soil in Korea. They were subjected to analysis using polyphasic taxonomy. Strains KSL-17, KSL-18 and KSL-19 grew optimally at pH 8·0 and 28 °C and in the presence of 0·5 % (w/v) NaCl. They were characterized chemotaxonomically as having -2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H) as the predominant menaquinone and iso-C as the major fatty acid. Their DNA G+C contents were in the range 74·6–74·8 mol%. Strains KSL-17, KSL-18 and KSL-19 were identical in terms of their 16S rRNA gene sequences and exhibited a mean level of DNA–DNA relatedness of 85–90 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains KSL-17, KSL-18 and KSL-19 formed a distinct phylogenetic lineage within the genus . The levels of 16S rRNA gene sequence similarity between the three strains and the type strains of species were in the range 92·6–95·2 %. On the basis of phenotypic, genetic and phylogenetic data, strains KSL-17 (=KCTC 19039=DSM 16315), KSL-18 and KSL-19 should be classified as members of a novel species of the genus , for which the name sp. nov. is proposed.

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2006-01-01
2024-11-10
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References

  1. Collins, M. D., Dorsch, M. & Stackebrandt, E.(1989). Transfer of Pimelobacter tumescens to Terrabacter gen. nov. as Terrabacter tumescens comb. nov. and of Pimelobacter jensenii to Nocardioides as Nocardioides jensenii comb. nov. Int J Syst Bacteriol 39, 1–6.[CrossRef] [Google Scholar]
  2. Collins, M. D., Cockcroft, S. & Wallbanks, S.(1994). Phylogenetic analysis of a new ll-diaminopimelic acid-containing coryneform bacterium from herbage, Nocardioides plantarum sp. nov. Int J Syst Bacteriol 44, 523–526.[CrossRef] [Google Scholar]
  3. Komagata, K. & Suzuki, K.(1987). Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–203. [Google Scholar]
  4. Lawson, P. A., Collins, M. D., Schumann, P., Tindall, B. J., Hirsch, P. & Labrenz, M.(2000). New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella lacustris sp. nov. Syst Appl Microbiol 23, 219–229.[CrossRef] [Google Scholar]
  5. Miller, E. S., Woese, C. R. & Brenner, S.(1991). Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov. Int J Syst Bacteriol 41, 363–368.[CrossRef] [Google Scholar]
  6. Park, Y.-H., Yoon, J.-H., Shin, Y. K., Suzuki, K.-I., Kudo, T., Seino, A., Kim, H.-J., Lee, J.-S. & Lee, S. T.(1999). Classification of ‘Nocardioides fulvus’ IFO 14399 and Nocardioides sp. ATCC 39419 in Kribbella gen. nov., as Kribbella flavida sp. nov. and Kribbella sandramycini sp. nov. Int J Syst Bacteriol 49, 743–752.[CrossRef] [Google Scholar]
  7. Prauser, H.(1976).Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 58–65.[CrossRef] [Google Scholar]
  8. Prauser, H.(1984).Nocardioides luteus spec. nov. Z Allg Microbiol 24, 647–648.[CrossRef] [Google Scholar]
  9. Prauser, H.(1989). Genus Nocardioides Prauser 1976 . In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2371–2375. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  10. Schippers, A., Schumann, P. & Spröer, C.(2005).Nocardioides oleivorans sp. nov., a novel crude oil-degrading bacterium. Int J Syst Evol Microbiol 55, 1501–1504.[CrossRef] [Google Scholar]
  11. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  12. Suzuki, K. & Komagata, K.(1983).Pimelobacter gen. nov., a new genus of coryneform bacteria with ll-diaminopimelic acid in the cell wall. J Gen Appl Microbiol 29, 59–71.[CrossRef] [Google Scholar]
  13. Tamura, T. & Yokota, A.(1994). Transfer of Nocardioides fastidiosa Collins and Stackebrandt 1989 to the genus Aeromicrobium as Aeromicrobium fastidiosum comb. nov. Int J Syst Bacteriol 44, 608–611.[CrossRef] [Google Scholar]
  14. Urzì, C., Salamone, P., Schumann, P. & Stackebrandt, E.(2000).Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. Int J Syst Evol Microbiol 50, 529–536.[CrossRef] [Google Scholar]
  15. Wang, Y. M., Zhang, Z. S., Xu, X. L., Ruan, J. S. & Wang, Y.(2001).Actinopolymorpha singaporensis gen. nov., sp. nov., a novel actinomycete from the tropical rainforest of Singapore. Int J Syst Evol Microbiol 51, 467–473. [Google Scholar]
  16. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  17. Yi, H. & Chun, J.(2004a).Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 1295–1299.[CrossRef] [Google Scholar]
  18. Yi, H. & Chun, J.(2004b).Nocardioides aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 2151–2154.[CrossRef] [Google Scholar]
  19. Yoon, J.-H., Rhee, S.-K., Lee, J.-S., Park, Y.-H. & Lee, S. T.(1997).Nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column. Int J Syst Bacteriol 47, 933–938.[CrossRef] [Google Scholar]
  20. Yoon, J.-H., Cho, Y.-G., Lee, S. T., Suzuki, K.-I., Nakase, T. & Park, Y.-H.(1999).Nocardioides nitrophenolicus sp. nov., a p-nitrophenol-degrading bacterium. Int J Syst Bacteriol 49, 675–680.[CrossRef] [Google Scholar]
  21. Yoon, J.-H., Kim, I.-G., Kang, K. H., Oh, T.-K. & Park, Y.-H.(2004).Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. Int J Syst Evol Microbiol 54, 71–75.[CrossRef] [Google Scholar]
  22. Yoon, J.-H., Kim, I.-G., Lee, M.-H., Lee, C.-H. & Oh, T.-K.(2005a).Nocardioides alkalitolerans sp. nov., isolated from an alkaline serpentinite soil in Korea. Int J Syst Evol Microbiol 55, 809–814.[CrossRef] [Google Scholar]
  23. Yoon, J.-H., Kim, I.-G., Lee, M.-H. & Oh, T.-K.(2005b).Nocardioides kribbensis sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 55, 1611–1614.[CrossRef] [Google Scholar]
  24. Yoon, J.-H., Lee, C.-H. & Oh, T.-K.(2005c).Nocardioides dubius sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 55, 2209–2212.[CrossRef] [Google Scholar]
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vol. , part 1, pp. 271-275

Cellular fatty acid content of strains KSL-17 , KSL-18 and KSL-19. [PDF](22 KB)



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