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Volume 56, Issue 3

sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China Free

Abstract

Three Gram-positive, moderately halophilic, spore-forming rods, designated XH-63, XH-62 and EJ-15, were isolated from two salt lakes located near Xilin Hot and Ejinor, in Inner Mongolia, China. The strains were strictly aerobic and motile, with spherical, terminal and deforming endospores. They grew at pH 5·0–9·0 (optimal growth at pH 7·5), between 4 and 45 °C (optimal growth at 37 °C) and at salinities of 1–20 % (w/v) total salts, growing optimally at 10 % (w/v) salts. They had -diaminopimelic acid in the cell wall peptidoglycan and DNA G+C contents of 36·1–37·1 mol%. The polar lipid pattern of strain XH-63, selected as the representative strain, consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and a phospholipid and two amino phospholipids of unknown structure. This strain possessed anteiso-C and anteiso-C as the major fatty acids (altogether representing 72·5 % of total) and MK-7 as the major menaquinone. 16S rRNA gene analysis of the three strains showed that they were within the cluster, with highest sequence similarity (95·4–95·8 %) with . Based on a combination of phenotypic, chemotaxonomic and phylogenetic features, it is proposed that the three isolates represent a novel species of the genus , sp. nov. The type strain is strain XH-63 (=CCM 7326=AS 1.4250=CECT 7097).

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2006-03-01
2024-05-10
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References

  1. Arahal D. R., Ventosa A. 2002; Moderately halophilic and halotolerant species of Bacillus and related genera. In Applications and Systematics of Bacillus and Relatives pp  83–99 Edited by Berkeley R., Heyndrickx M., Logan N., De Vos P. Oxford: Blackwell;
     [Google Scholar]
  2. Arahal D. R., Márquez M. C., Volcani B. E., Schleifer K. H., Ventosa A. 1999; Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J Syst Bacteriol 49:521–530 [CrossRef]
     [Google Scholar]
  3. Arahal D. R., Márquez M. C., Volcani B. E., Schleifer K. H., Ventosa A. 2000; Reclassification of Bacillus marismortui as Salibacillus marismortui comb. nov. Int J Syst Evol Microbiol 50:1501–1503 [CrossRef]
     [Google Scholar]
  4. Arahal D. R., García M. T., Ludwig W., Schleifer K. H., Ventosa A. 2001; Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51:1443–1448
     [Google Scholar]
  5. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13:202–206
     [Google Scholar]
  6. De Ley J., Tijtgat R. 1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36:461–474 [CrossRef]
     [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
     [Google Scholar]
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
     [Google Scholar]
  9. García M. T., Ventosa A., Ruiz-Berraquero F., Kocur M. 1987; Taxonomic study and amended description of Vibrio costicola . Int J Syst Bacteriol 37:251–256 [CrossRef]
     [Google Scholar]
  10. García M. T., Gallego V., Ventosa A., Mellado E. 2005; Thalassobacillus devorans gen. nov., sp. nov. a moderately halophilic, phenol-degrading, Gram-positive bacterium. Int J Syst Evol Microbiol 55:1789–1795 [CrossRef]
     [Google Scholar]
  11. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A. 1998; Virgibacillus : a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus . Int J Syst Bacteriol 48:99–106 [CrossRef]
     [Google Scholar]
  12. Heyrman J., Logan N. A., Busse H.-J., Balcaen A., Lebbe L., Rodriguez-Diaz M., Swings J., De Vos P. 2003; Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus , as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus . Int J Syst Evol Microbiol 53:501–511 [CrossRef]
     [Google Scholar]
  13. Ishikawa M., Ishizaki S., Yamamoto Y., Yamasato K. 2002; Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga. J Gen Appl Microbiol 48269–279 [CrossRef]
     [Google Scholar]
  14. Jeon C. O., Lim J.-M., Lee J.-C., Lee G. S., Lee J.-M., Xu L.-H., Jiang C.-L., Kim C.-J. 2005a; Lentibacillus salarius sp. nov., isolated from saline sediment in China, and emended description of the genus Lentibacillus . Int J Syst Evol Microbiol 55:1339–1343 [CrossRef]
     [Google Scholar]
  15. Jeon C. O., Lim J.-M., Lee J.-M., Xu L.-H., Jiang C.-L., Kim C.-J. 2005b; Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55:1891–1896 [CrossRef]
     [Google Scholar]
  16. Johnson J. L. 1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology . pp  655–681 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  17. Lawson P. A., Deutsch C. E., Collins M. D. 1996; Phylogenetic characterization of a novel salt-tolerant Bacillus species: description of Bacillus dipsosauri sp. nov.. J Appl Bacteriol 81:109–112 [CrossRef]
     [Google Scholar]
  18. Lim J.-M., Jeon C. O., Song S. M., Kim C.-J. 2005a; Pontibacillus chungwhensis gen. nov., sp. nov. a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55:165–170 [CrossRef]
     [Google Scholar]
  19. Lim J.-M., Jeon C. O., Park D.-J., Kim H.-R., Yoon B.-J., Kim C.-J. 2005b; Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus . Int J Syst Evol Microbiol 55:1027–1031 [CrossRef]
     [Google Scholar]
  20. Lu J., Nogi Y., Takami H. 2001; Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205:291–297 [CrossRef]
     [Google Scholar]
  21. Ludwig W., Strunk O. 1996; arb – a software environment for sequence data. http://www.mikro.biologie.tu-muenchen.de/pub/ARB/documentation/ARB.ps
     [Google Scholar]
  22. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
     [Google Scholar]
  23. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
     [Google Scholar]
  24. Mellado E., Moore E. R. B., Nieto J. J., Ventosa A. 1995; Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui , Volcaniella eurihalina , and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45:712–716 [CrossRef]
     [Google Scholar]
  25. Nielsen P., Rainey F. A., Outtrup H., Priest F. G., Fritze D. 1994; Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus . FEMS Microbiol Lett 117:61–66 [CrossRef]
     [Google Scholar]
  26. Owen R. J., Hill L. R. 1979; The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists , 2nd edn. pp  217–296 Edited by Skinner F. A., Lovelock D. W. London: Academic Press;
     [Google Scholar]
  27. Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A. 1984; Deleya halophila , a new species of moderately halophilic bacteria. Int J Syst Bacteriol 40:287–292
     [Google Scholar]
  28. Ren P.-G., Zhou P.-J. 2005a; Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. Int J Syst Evol Microbiol 55:95–99 [CrossRef]
     [Google Scholar]
  29. Ren P.-G., Zhou P.-J. 2005b; Salinibacillus aidingensis gen nov., sp. nov. and Salinibacillus kushneri sp. nov., moderately halophilic bacteria isolated from a neutral saline lake in Xin-Jiang, China. Int J Syst Evol Microbiol 55949–953 [CrossRef]
     [Google Scholar]
  30. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  31. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
     [Google Scholar]
  32. Schlesner H., Lawson P. A., Collins M. D., Weiss N., Wehmeyer U., Völker H., Thomm M. 2001; Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51:425–431
     [Google Scholar]
  33. Spring S., Ludwig W., Márquez M. C., Ventosa A., Schleifer K. H. 1996; Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
     [Google Scholar]
  34. Stackebrandt E., Liesack W. 1993; Nucleic acids and classification. In Handbook of New Bacterial Systematics pp  152–189 Edited by Goodfellow M., O'Donnell A. G. London: Academic Press;
     [Google Scholar]
  35. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
     [Google Scholar]
  36. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
     [Google Scholar]
  37. Ventosa A., Ramos-Cormenzana A., Kocur M. 1983; Moderately halophilic Gram-positive cocci from hypersaline environments. Syst Appl Microbiol 4:546–570
     [Google Scholar]
  38. Ventosa A., García M. T., Kamekura M., Onishi H., Ruiz-Berraquero F. 1989; Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Syst Appl Microbiol 12:162–165 [CrossRef]
     [Google Scholar]
  39. Ventosa A., Nieto J. J., Oren A. 1998; Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62:504–544
     [Google Scholar]
  40. Wainø M., Tindall B. J., Schumann P., Ingvorsen K. 1999; Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49:821–831 [CrossRef]
     [Google Scholar]
  41. Yoon J.-H., Weiss N., Lee K.-C., Lee I.-S., Kang K. H., Park Y.-H. 2001; Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov. Int J Syst Evol Microbiol 51, 2087–2093 [CrossRef]
     [Google Scholar]
  42. Yoon J.-H., Kang K. H., Park Y.-H. 2002; Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 52:2043–2048 [CrossRef]
     [Google Scholar]
  43. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53:687–693 [CrossRef]
     [Google Scholar]
  44. Zhilina T. N., Garnova E. S., Tourova T. P., Kostrikina N. A., Zavarzin G. A. 2001; Amphibacillus fermentum sp. nov. and Amphibacillus tropicus sp. nov., new alkaliphilic, facultatively anaerobic, saccharolytic bacilli from Lake Magadi. Microbiology (English translation of Mikrobiologiia) 70:711–722
     [Google Scholar]
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Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China
Int J Syst Evol Microbiol 56, 599 (2006); https://doi.org/10.1099/ijs.0.63971-0
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