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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 56, Issue 1

sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an Indian tropical leguminous plant Free

Abstract

The bacterial strain SJT, along with 15 other mesophilic, neutrophilic and facultatively sulfur-oxidizing chemolithotrophic isolates, was isolated by enrichment on reduced sulfur compounds as the sole energy and electron source from soils immediately adjacent to the roots of , a slender leguminous herb of the Lower Gangetic plains of India. Strain SJT was able to oxidize thiosulfate and elemental sulfur for chemolithoautotrophic growth. 16S rRNA and gene sequence-based phylogenetic analyses showed that the Gram-negative rod-shaped bacterium belonged to the genus and was most closely related to , , and . Unequivocally low 16S rRNA (<97 %) and (⩽88 %) gene sequence similarities to all existing species of the most closely related genera, a unique fatty acid profile, a distinct G+C content (59·6 mol%) and phenotypic characteristics all suggested that strain SJT represents a novel species. DNA–DNA hybridization and SDS-PAGE analysis of whole-cell proteins also confirmed the taxonomic uniqueness of SJT. It is therefore proposed that isolate SJT (=LMG 22697=MTCC 7001) be classified as the type strain of a novel species, sp. nov.

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2006-01-01
2025-03-24
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Mesorhizobium thiogangeticum sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an Indian tropical leguminous plant
Int J Syst Evol Microbiol 56, 91 (2006); https://doi.org/10.1099/ijs.0.63967-0
/content/journal/ijsem/10.1099/ijs.0.63967-0
/content/journal/ijsem/10.1099/ijs.0.63967-0
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Phenogram of sp. nov. SJT and related strains deduced by comparing their available phenotypic characteristics. [PDF](40 KB)

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Coomassie blue-stained SDS-PAGE gel of whole-cell-protein preparations from sp. nov. SJT (duplicate samples in lanes 1 and 2) in comparison with its phylogenetic relatives KCT001 (lane 3), LMG 17826 t2 (4), LMG 18932 (5), LMG 19008 (6) and LMG 11892 (7).

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Fatty acid profile of sp. nov. SJT in comparison with related taxa. [PDF](26 KB)

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