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A Gram-positive actinobacterium, strain RKJ300T, capable of utilizing p-nitrophenol and 2,4-dinitrophenol, was isolated from a pesticide-contaminated site in India. The morphological and chemotaxonomic properties of the isolate were typical of members of the genus Rhodococcus. The DNA G+C content was 72 mol%. Strain RKJ300T exhibited the highest level of sequence similarity with Rhodococcus wratislaviensis NCIMB 13082T (99.3 %), followed by Rhodococcus opacus DSM 43205T (98.8 %), Rhodococcus percolatus MBS1T (98.6 %) and Rhodococcus koreensis DNP505T (98.1 %). The low levels of DNA–DNA relatedness (49–58 %) with the above micro-organisms, and the differences in the biochemical and physiological properties, suggest that strain RKJ300T should be classified within a novel species of the genus Rhodococcus, for which the name Rhodococcus imtechensis sp. nov. is proposed. The type strain is RKJ300T (=MTCC 7085T=JCM 13270T).
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