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Abstract

Two strictly anaerobic bacterial strains (YUAN-3 and X-29) were isolated from anaerobic activated sludge of molasses wastewater in a continuous stirred-tank reactor. The strains were Gram-positive, non-spore-forming, mesophilic and motile. Cells were regular rods (0·4–0·8×1·5–8·0 μm) and occurred singly, in pairs and sometimes in chains of up to eight. Autoaggregative and autofluorescent growth of strain YUAN-3 and non-aggregative growth of strain X-29 were observed at 20–44 °C and pH 3·5–9·0. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. Fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids iso-C (44·18 %) and iso-C (26·67 %). The DNA G+C contents of strains YUAN-3 and X-29 were 47·8 and 49·0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a novel phyletic sublineage within the rRNA cluster, with <92 % 16S rRNA gene sequence similarity to currently known species. On the basis of polyphasic evidence from this study, it is proposed that the unknown bacterium should be classified in a new genus as a novel species, gen. nov., sp. nov. The type strain of is YUAN-3 (=JCM 12961=CGMCC 1.5033).

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2006-04-01
2020-01-26
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vol. , part 4, pp. 755 - 760

Colony and electron micrographs of cells of strain YUAN-3 . (a) Colony on agar medium. Bar, 1 mm. (b) TEM of cell in liquid culture. Bar, 0.5 µm. (c) TEM of cell on agar medium. Bar, 0.5 µm (C, capsule). (d) Ultrathin section EM (CW, cell wall; CM, cytoplasmic membrane; PHB, poly-β-hydroxybutyrate inclusion body; PP, polyphosphate granules). Bar, 0.1 µm.



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