Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 56, Issue 1

gen. nov., sp. nov., a novel member of the family isolated from a marine sponge in the Bahamas, and emended description of Free

Abstract

A bacterial strain, UST030701-156, was isolated from a marine sponge in the Bahamas. Strain UST030701-156 was orange-pigmented, Gram-negative, rod-shaped with tapered ends, slowly motile by gliding and strictly aerobic. The predominant fatty acids were a15 : 0, i15 : 0, i15 : 0 3-OH, i17 : 0 3-OH, i17 : 19 and summed feature 3, comprising i15 : 0 2-OH and/or 16 : 17. MK-6 was the only respiratory quinone. Flexirubin-type pigments were not produced. Phylogenetic analysis based on 16S rRNA gene sequences placed UST030701-156 within a distinct lineage in the family , with 93·3 % sequence similarity to the nearest neighbour, . The DNA G+C content of UST030701-156 was 41·0 mol% and was much higher than that of (33·6 mol%). Strain UST030701-156 can be distinguished from other members of the by means of a number of chemotaxonomic and phenotypic characteristics. It is proposed, therefore, that UST030701-156 represents a novel taxon designated gen. nov., sp. nov. The type strain is UST030701-156 (=NRRL B-41138=JCM 13191). Carbon-source utilization by was re-examined and an emended description is therefore included.

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63908-0
2006-01-01
2025-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/1/181.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63908-0&mimeType=html&fmt=ahah

References

  1. Acar, J. F.(1980). The disc susceptibility test. In Antibiotics in Laboratory Medicine, pp. 24–54. Edited by V. Lorian. Baltimore: Williams & Wilkins.
  2. Baumann, P. & Baumann, L.(1988). The marine gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes. In The Prokaryotes, vol. 1, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. Schlegel. Berlin: Springer.
  3. Bernardet, J. F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  4. Bernardet, J. F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  5. Bowman, J. P.(2000). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868. [Google Scholar]
  6. Bowman, J. P., McCammon, S. A., Lewis, T., Skerratt, J. H., Brown, J. L., Nichols, D. S. & McMeekin, T. A.(1998).Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense (Dobson et al. 1993) as Psychroflexus gondwanense gen. nov., comb. nov. Microbiology 144, 1601–1609.[CrossRef] [Google Scholar]
  7. Collins, M. D.(1994). Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics, pp. 265–310. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  8. Donachie, S. P., Bowman, J. P. & Alam, M.(2004).Psychroflexus tropicus sp. nov., an obligately halophilic Cytophaga–Flavobacterium–Bacteroides group bacterium from an Hawaiian hypersaline lake. Int J Syst Evol Microbiol 54, 935–940.[CrossRef] [Google Scholar]
  9. Isnansetyo, A. & Kamei, Y.(2003).Pseudoalteromonas phenolica sp. nov., a novel marine bacterium that produces phenolic anti-methicillin-resistant Staphylococcus aureus substances. Int J Syst Evol Microbiol 53, 583–588.[CrossRef] [Google Scholar]
  10. Ivanova, E. P., Alexeeva, Y. V., Flavier, S., Wright, J. P., Zhukova, N. V., Gorshkova, N. M., Mikhailov, V. V., Nicolau, D. V. & Christen, R.(2004).Formosa algae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 54, 705–711.[CrossRef] [Google Scholar]
  11. Johansen, J. E., Nielsen, P. & Sjoholm, C.(1999). Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov. Int J Syst Bacteriol 49, 1231–1240.[CrossRef] [Google Scholar]
  12. Lau, S. C. K., Tsoi, M. M. Y., Li, X., Plakhotnikova, I., Wu, M., Wong, P.-K. & Qian, P.-Y.(2004).Loktanella hongkongensis sp. nov., a novel member of the α-Proteobacteria originating from marine biofilms in Hong Kong waters. Int J Syst Evol Microbiol 54, 2281–2284.[CrossRef] [Google Scholar]
  13. Lau, S. C. K., Tsoi, M. M. Y., Li, X. & 7 other authors(2005a).Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas. Int J Syst Evol Microbiol 55, 1589–1592.[CrossRef] [Google Scholar]
  14. Lau, S. C. K., Tsoi, M. M. Y., Li, X., Plakhotnikova, I., Dobretsov, S., Wong, P.-K., Pawlik, J. R. & Qian, P.-Y.(2005b).Nonlabens tegetincola gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a microbial mat in a subtropical estuary. Int J Syst Evol Microbiol 55, 2279–2283.[CrossRef] [Google Scholar]
  15. Ludwig, W., Strunk, O., Westram, R. & 29 other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  16. MacDonell, M. T., Singleton, F. L. & Hood, M. A.(1982). Diluent composition for use of API 20E characterizing marine and estuarine bacteria. Appl Environ Microbiol 44, 423–427. [Google Scholar]
  17. McCammon, S. A. & Bowman, J. P.(2000). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev., and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063.[CrossRef] [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  19. Nedashkovskaya, O. I., Kim, S. B., Han, S. K. & 7 other authors(2003).Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. Int J Syst Evol Microbiol 53, 1967–1971.[CrossRef] [Google Scholar]
  20. Nedashkovskaya, O. I., Suzuki, M., Vancanneyt, M., Cleenwerck, I., Zhukova, N. V., Vysotskii, M. V., Mikhailov, V. V. & Swings, J.(2004).Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus. Int J Syst Evol Microbiol 54, 1107–1110.[CrossRef] [Google Scholar]
  21. Nedashkovskaya, O. I., Kim, S. B., Han, S. K. & 9 other authors(2005a).Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 55, 49–55.[CrossRef] [Google Scholar]
  22. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Mikhailov, V. V., Bae, K. S. & Kim, I. S.(2005b).Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 55, 235–237.[CrossRef] [Google Scholar]
  23. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Frolova, G. M., Mikhailov, V. V., Bae, K. S., Lee, D. H. & Kim, I. S.(2005c).Gramella echinicola gen. nov., sp. nov., a novel halophilic bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 55, 391–394.[CrossRef] [Google Scholar]
  24. Nedashkovskaya, O. I., Vancanneyt, M., Dawyndt, P. & 9 other authors(2005d). Reclassification of [Cytophaga] marinoflava Reichenbach 1989 as Leeuwenhoekiella marinoflava gen. nov., comb. nov. and description of Leeuwenhoekiella aequorea sp. nov. Int J Syst Evol Microbiol 55, 1033–1038.[CrossRef] [Google Scholar]
  25. Neu, B., Voigt, A., Mitlohner, R. & 7 other authors(2001). Biological cells as templates for hollow microcapsules. J Microencapsul 18, 385–395.[CrossRef] [Google Scholar]
  26. Norris, J. R., Ribbons, D. W. & Varma, A. K. (editors)(1985).Methods in Microbiology, vol. 18. London: Academic Press.
  27. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characteristics. In Methods for General and Molecular Biology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  28. Steyn, P. L., Segers, P., Vancanneyt, M., Sandra, P., Kersters, K. & Joubert, J. J.(1998). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. Proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48, 165–177.[CrossRef] [Google Scholar]
  29. Yoon, J.-H., Kang, S.-J., Lee, C.-H. & Oh, T.-K.(2006).Donghaena dokdonensis gen. nov., sp. nov., isolated from sea water. Int J Syst Evol Microbiol 56, 187–191.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63908-0
Loading
Stenothermobacter spongiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a marine sponge in the Bahamas, and emended description of Nonlabens tegetincola
Int J Syst Evol Microbiol 56, 181 (2006); https://doi.org/10.1099/ijs.0.63908-0
/content/journal/ijsem/10.1099/ijs.0.63908-0
/content/journal/ijsem/10.1099/ijs.0.63908-0
Loading

Data & Media loading...

Supplements

Cells of strain UST030701-156 visualized using a scanning electron microscope. Bar, 1 µm.

IMAGE

[PDF file of Supplementary Tables S1-S3](37 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error