1887

Abstract

Two actinomycete strains, 32U-2 and 32U-4, were isolated from a prairie soil in Minnesota and subjected to characterization by means of polyphasic taxonomy. The 16S rRNA gene sequences were determined following PCR amplification and cloning. A phylogenetic analysis, based on comparative analysis of 16S rRNA gene sequences, indicated that the organisms consistently formed a well-separated, distinct sub-branch within the radiation of the genus of the family . The levels of 16S rRNA sequence similarity between the isolates and the type strains of recognized species ranged from 94·1 to 97·9 %. The highest levels of sequence similarity were found between the isolates and (97·6–97·9 %), and (97·3–97·6 %) and (97·2–97·5 %). Chemotaxonomic characteristics supported the phylogenetic relationships between the organisms and members of the genus . However, a broad range of phenotypic and genetic data revealed that the isolates should be classified as novel species of the genus , for which the name sp. nov. is proposed. The type strain is 32U-2 (=KCCM 42246=NRRL B-24435).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63907-0
2006-01-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/1/265.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63907-0&mimeType=html&fmt=ahah

References

  1. Brosius, J., Palmer, J. L., Kennedy, J. P. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4480.[CrossRef]
    [Google Scholar]
  2. Embley, T. M., Smida, J. & Stackebrandt, E. ( 1988; ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and the description of Pseudonocardiaceae fam. nov. Syst Appl Microbiol 11, 44–52.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  5. Henssen, A., Kothe, H. W. & Kroppenstedt, R. M. ( 1987; ). Transfer of Pseudonocardia azurea and “Pseudonocardia fastidiosa” to the genus Amycolatopsis, with emended species description. Int J Syst Bacteriol 37, 292–295.[CrossRef]
    [Google Scholar]
  6. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  7. Lechevalier, M. P., Prauser, H., Labeda, D. P. & Ruan, J.-S. ( 1986; ). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36, 29–37.[CrossRef]
    [Google Scholar]
  8. Lee, S. D. & Hah, Y. C. ( 2001; ). Amycolatopsis albidoflavus sp. nov. Int J Syst Evol Microbiol 51, 645–650.
    [Google Scholar]
  9. Lee, S. D., Kim, E. S. & Hah, Y. C. ( 2000; ). Phylogenetic analysis of the genera Pseudonocardia and Actinobispora based on 16S rDNA sequence studies. FEMS Microbiol Lett 182, 125–129.[CrossRef]
    [Google Scholar]
  10. MacFaddin, J. F. ( 1981; ). Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  11. Mertz, F. P. & Yao, R. C. ( 1993; ). Amycolatopsis alba sp. nov., isolated from soil. Int J Syst Bacteriol 43, 715–720.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Minnikin, D. E. M. ( 1988; ). Isolation and purification of mycobacterial wall lipids. In Bacterial Cell Surface Techniques, pp. 125–135. Edited by I. C. Hancock & I. R. Poxton. Chichester: Wiley.
  14. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1977; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88, 200–204.
    [Google Scholar]
  15. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  16. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  17. Saddler, G. S., Tavecchia, P., Lociuro, S., Zanol, M., Colombo, E. & Selva, E. ( 1991; ). Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14, 185–191.[CrossRef]
    [Google Scholar]
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  20. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  22. Uchida, K. & Aida, K. ( 1984; ). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef]
    [Google Scholar]
  23. Warwick, S., Bowen, T., McVeigh, H. P. & Embley, T. M. ( 1994; ). A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia. Int J Syst Bacteriol 44, 293–299.[CrossRef]
    [Google Scholar]
  24. Yassin, A. F., Haggenei, B., Budzikiewicz, H. & Schaal, K. P. ( 1993; ). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast bombardment-mass spectrometry to the structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43, 414–420.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63907-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63907-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error