1887

Abstract

A Gram-positive, aerobic, non-motile strain, H2.16B, isolated from the midgut of the mosquito was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strain H2.16B was shown to belong to the genus , being most closely related to (98·3 %), (98·5 %) and (98·5 %). Chemotaxonomic data (-diaminopimelic acid as the diagnostic diamino acid in the cell wall and major fatty acids of iso-C, C 8 and C) supported the allocation of the strain to the genus . The results of DNA–DNA hybridization and physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain H2.16B from closely related species. Thus, H2.16B represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H2.16B (=CCUG 49715=CIP 108728).

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2006-02-01
2019-12-13
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References

  1. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  2. Imamura, Y., Ikeda, M., Yoshida, S. & Kuraishi, H. ( 2000; ). Janibacter brevis sp. nov., a new trichloroethylene-degrading bacterium isolated from polluted environments. Int J Syst Evol Microbiol 50, 1899–1903.
    [Google Scholar]
  3. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  4. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  5. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J. ( 2003; ). Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef]
    [Google Scholar]
  6. Kroppenstedt, R. M. ( 1985; ). Fatty acid menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  7. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  8. Lang, E., Kroppenstedt, R. M., Swiderski, J., Schumann, P., Ludwig, W., Schmid, A. & Weiss, N. ( 2003; ). Emended description of Janibacter terrae, including ten dibenzofuran-degrading strains and Janibacter brevis as its later heterotypic synonym. Int J Syst Evol Microbiol 53, 1999–2005.[CrossRef]
    [Google Scholar]
  9. Lindh, J. M., Terenius, O. & Faye, I. ( 2005; ). 16S rRNA gene-based identification of midgut bacteria from field-caught Anopheles gambiae sensu lato and A. funestus mosquitoes reveals new species related to known insect symbionts. Appl Environ Microbiol 71, 7217–7223.[CrossRef]
    [Google Scholar]
  10. Ludwig, W., Strunk, O., Westram, R. & 29 other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  11. Martin, K., Schumann, P., Rainey, F. A., Schuetze, B. & Groth, I. ( 1997; ). Janibacter limosus gen. nov., sp. nov., a new actinomycete with meso-diaminopimelic acid in the cell wall. Int J Syst Bacteriol 47, 529–534.[CrossRef]
    [Google Scholar]
  12. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  13. Yoon, J.-H., Lee, K.-C., Kang, S.-S., Kho, Y. H., Kang, K. H. & Park, Y.-H. ( 2000; ). Janibacter terrae sp. nov., a bacterium isolated from soil around a wastewater treatment plant. Int J Syst Evol Microbiol 50, 1821–1827.
    [Google Scholar]
  14. Yoon, J.-H., Lee, B. H., Yeo, S.-H. & Choi, J.-E. ( 2004; ). Janibacter melonis sp. nov., isolated from abnormally spoiled oriental melon in Korea. Int J Syst Evol Microbiol 54, 1975–1980.[CrossRef]
    [Google Scholar]
  15. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R. ( 1998; ). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef]
    [Google Scholar]
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