1887

Abstract

A novel anaerobic, moderately thermophilic bacterium, strain Cas60314, was isolated from hot oil-well production water obtained from an oil reservoir in the North Sea. The cells were Gram-negative, motile, straight rods. The salinity and pH growth optima were 2.0–3.0 % NaCl and 6.5–7.0, respectively. The optimum temperature was 58 °C. Strain Cas60314 had a fermentative type of metabolism and utilized proteinous substrates, some single amino acids and a limited number of organic acids, but not sugars, fatty acids or alcohols. Cystine and elemental sulfur were reduced to sulfide. The G+C content of the DNA was 46.6 mol%. On the basis of phenotypic and phylogenetic features, it is proposed that this isolate represents a novel genus and species with the name gen. nov., sp. nov. within the family . The proposed type strain is strain Cas60314 (=DSM 17291=ATTC BAA-1197).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63894-0
2006-07-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/7/1539.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63894-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J. H., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Baena, S., Fardeau, M. L., Labat, M., Ollivier, B., Thomas, P., Garcia, J. L. & Patel, B. K. C. ( 1998; ). Aminobacterium colombiense gen. nov. sp. nov., an amino acid-degrading anaerobe isolated from anaerobic sludge. Anaerobe 4, 241–250.[CrossRef]
    [Google Scholar]
  3. Baena, S., Fardeau, M. L., Ollivier, B., Labat, M., Thomas, P., Garcia, J. L. & Patel, B. K. C. ( 1999; ). Aminomonas paucivorans gen. nov., sp. nov., a mesophilic anaerobic, amino-acid-utilizing bacterium. Int J Syst Bacteriol 49, 975–982.[CrossRef]
    [Google Scholar]
  4. Baena, S., Fardeau, M. L., Labat, M., Ollivier, B., Garcia, J. L. & Patel, B. K. C. ( 2000; ). Aminobacterium mobile sp. nov., a new anaerobic amino-acid-degrading bacterium. Int J Syst Evol Microbiol 50, 259–264.[CrossRef]
    [Google Scholar]
  5. Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R. & Wolfe, R. S. ( 1979; ). Methanogens: re-evaluation of a unique biological group. Microbiol Rev 43, 260–296.
    [Google Scholar]
  6. Benson, D. A., Karsch-Mizrachi, I., Lipman, D. J., Ostell, J. & Wheeler, D. L. ( 2005; ). GenBank. Nucleic Acids Res 33, D34–D38.[CrossRef]
    [Google Scholar]
  7. Birkeland, N. K. ( 2004; ). The microbial diversity of deep subsurface oil reservoirs. In Petroleum Biotechnology: Developments and Perspectives, pp. 385–403. Edited by R. Vazquez-Duhalt & R. Quintero-Ramirez. Amsterdam: Elsevier.
  8. Cheng, G. S., Plugge, C. M., Roelofsen, W., Houwen, F. P. & Stams, A. J. M. ( 1992; ). Selenomonas acidaminovorans sp. nov., a versatile thermophilic proton-reducing anaerobe able to grow by decarboxylation of succinate to propionate. Arch Microbiol 157, 169–175.
    [Google Scholar]
  9. Cline, J. D. ( 1969; ). Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 14, 454–458.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  11. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 211–232. Edited by H. N. Munro. New York: Academic Press.
  12. Lien, T. & Beeder, J. ( 1997; ). Desulfobacter vibrioformis sp. nov., a sulfate reducer from a water–oil separation system. Int J Syst Bacteriol 47, 1124–1128.[CrossRef]
    [Google Scholar]
  13. Lien, T., Madsen, M., Rainey, F. A. & Birkeland, N. K. ( 1998; ). Petrotoga mobilis sp. nov., from a North Sea oil-production well. Int J Syst Bacteriol 48, 1007–1013.[CrossRef]
    [Google Scholar]
  14. Loffler, F. E., Sun, Q., Li, J. R. & Tiedje, J. M. ( 2000; ). 16S rRNA gene-based detection of tetrachloroethene-dechlorinating Desulfuromonas and Dehalococcoides species. Appl Environ Microbiol 66, 1369–1374.[CrossRef]
    [Google Scholar]
  15. Lovley, D. R. & Phillips, E. J. P. ( 1986; ). Organic matter mineralization with the reduction of ferric iron in anaerobic sediments. Appl Environ Microbiol 51, 683–689.
    [Google Scholar]
  16. Magot, M., Caumette, P., Desperrier, J. M., Matheron, R., Dauga, C., Grimont, F. & Carreau, L. ( 1992; ). Desulfovibrio longus sp. nov., a sulfate-reducing bacterium isolated from an oil-producing well. Int J Syst Bacteriol 42, 398–403.[CrossRef]
    [Google Scholar]
  17. Magot, M., Ravot, G., Campaignolle, X., Ollivier, B., Patel, B. K. C., Fardeau, M. L., Thomas, P., Crolet, J. L. & Garcia, J. L. ( 1997; ). Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a new anaerobic, slightly halophilic, thiosulfate-reducing bacterium from corroding offshore oil wells. Int J Syst Bacteriol 47, 818–824.[CrossRef]
    [Google Scholar]
  18. Magot, M., Ollivier, B. & Patel, B. K. C. ( 2000; ). Microbiology of petroleum reservoirs. Antonie van Leeuwenhoek 77, 103–116.[CrossRef]
    [Google Scholar]
  19. Menes, R. J. & Muxi, L. ( 2002; ). Anaerobaculum mobile sp. nov., a novel anaerobic, moderately thermophilic, peptide-fermenting bacterium that uses crotonate as an electron acceptor, and emended description of the genus Anaerobaculum. Int J Syst Evol Microbiol 52, 157–164.
    [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. ( 1989; ). Precise measurement of the G+C content of deoxyribonucelic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  21. Miranda-Tello, E., Fardeau, M. L., Sepulveda, J., Fernandez, L., Cayol, J. L., Thomas, P. & Ollivier, B. ( 2003; ). Garciella nitratireducens gen. nov., sp nov., an anaerobic, thermophilic, nitrate- and thiosulfate-reducing bacterium isolated from an oilfield separator in the Gulf of Mexico. Int J Syst Evol Microbiol 53, 1509–1514.[CrossRef]
    [Google Scholar]
  22. Nazina, T. N., Ivanova, A. E., Kanchaveli, L. P. & Rozanova, E. P. ( 1988; ). A new spore-forming thermophilic methylotrophic sulfate-reducing bacterium, Desulfotomaculum kuznetsovii sp. nov. Microbiology (English translation of Mikrobiologiia)57, 659–663.
    [Google Scholar]
  23. Nilsen, R. K., Torsvik, T. & Lien, T. ( 1996; ). Desulfotomaculum thermocisternum sp. nov., a sulfate reducer isolated from a hot North Sea oil reservoir. Int J Syst Bacteriol 46, 397–402.[CrossRef]
    [Google Scholar]
  24. Orphan, V. J., Taylor, L. T., Hafenbradl, D. & Delong, E. F. ( 2000; ). Culture-dependent and culture-independent characterization of microbial assemblages associated with high-temperature petroleum reservoirs. Appl Environ Microbiol 66, 700–711.[CrossRef]
    [Google Scholar]
  25. Ravot, G., Magot, M., Fardeau, M. L., Patel, B. K. C., Thomas, P., Garcia, J. L. & Ollivier, B. ( 1999; ). Fusibacter paucivorans gen. nov., sp. nov., an anaerobic, thiosulfate-reducing bacterium from an oil-producing well. Int J Syst Bacteriol 49, 1141–1147.[CrossRef]
    [Google Scholar]
  26. Rees, G. N., Patel, B. K. C., Grassia, G. S. & Sheehy, A. J. ( 1997; ). Anaerobaculum thermoterrenum gen. nov., sp nov., a novel, thermophilic bacterium which ferments citrate. Int J Syst Bacteriol 47, 150–154.[CrossRef]
    [Google Scholar]
  27. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  28. Surkov, A. V., Dubinina, G. A., Lysenko, A. M., Glockner, F. O. & Kuever, J. ( 2001; ). Dethiosulfovibrio russensis sp. nov., Dethiosulfovibrio marinus sp. nov. and Dethiosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from ‘Thiodendron’ sulfur mats in different saline environments. Int J Syst Evol Microbiol 51, 327–337.
    [Google Scholar]
  29. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  30. Widdel, F., Kohring, G. W. & Mayer, F. ( 1983; ). Studies on dissimilatory sulfate-reducing bacteria that decompose fatty-acids. III. Characterization of the filamentous gliding Desulfonema limicola gen. nov., sp. nov., and Desulfonema magnum sp. nov. Arch Microbiol 134, 286–294.[CrossRef]
    [Google Scholar]
  31. Zavarzina, D. G., Zhilina, T. N., Tourova, T. P., Kuznetsov, B. B., Kostrikina, N. A. & Bonch-Osmolovskaya, E. A. ( 2000; ). Thermanaerovibrio velox sp. nov., a new anaerobic, thermophilic, organotrophic bacterium that reduces elemental sulfur, and emended description of the genus Thermanaerovibrio. Int J Syst Evol Microbiol 50, 1287–1295.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63894-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63894-0
Loading

Data & Media loading...

Supplements

vol. , part 7, pp. 1539-1545

The morphology and growth temperature profile of gen. nov., sp. nov. [PDF](37 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error