1887

Abstract

Two actinomycete strains, designated N7-3 and N4-6, were isolated from a natural cave on Jeju Island, Republic of Korea, by using a dilution method, and were subjected to physiological, chemical and molecular characterization. The nearly complete sequences of the 16S rRNA gene were aligned and compared with those of representatives of the genus . Phylogenetic analysis showed that the organisms belong to the family and formed two distinct lineages within the evolutionary radius of the genus . The chemotaxonomic and morphological properties support their classification in the genus . The 16S rRNA gene sequence data revealed that the closest relatives of strains N7-3 and N4-6 were (97·9 % similarity) and (98·7 % similarity), respectively. The combination of physiological and genetic data supported the observation that the organisms could be distinguished from each other and from established species of the genus . The names sp. nov. and sp. nov. are proposed for the two novel species, with N7-3 (=NRRL B-24427=JCM 13280) and N4-6 (=NRRL B-24428=JCM 13279) as the respective type strains.

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2006-03-01
2019-10-14
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References

  1. Brosius, J., Palmer, J. L., Kennedy, J. P. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4480.[CrossRef]
    [Google Scholar]
  2. Embley, T. M., Smida, J. & Stackebrandt, E. ( 1988; ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and the description of Pseudonocardiaceae fam. nov. Syst Appl Microbiol 11, 44–52.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1993; ). phylip (phylogenetic inference package), version 3.5. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Fitch, W. M. ( 1971; ). Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  7. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  8. Henssen, A., Kothe, H. W. & Kroppenstedt, R. M. ( 1987; ). Transfer of Pseudonocardia azurea and “Pseudonocardia fastidiosa” to the genus Amycolatopsis, with emended species description. Int J Syst Bacteriol 37, 292–295.[CrossRef]
    [Google Scholar]
  9. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  10. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  11. Lechevalier, M. P., Prauser, H., Labeda, D. P. & Ruan, J.-S. ( 1986; ). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36, 29–37.[CrossRef]
    [Google Scholar]
  12. Lee, S. D., Kim, E. S. & Hah, Y. C. ( 2000a; ). Phylogenetic analysis of the genera Pseudonocardia and Actinobispora based on the 16S ribosomal DNA sequences. FEMS Microbiol Lett 182, 125–129.[CrossRef]
    [Google Scholar]
  13. Lee, S. D., Kim, E. S., Roe, J.-H., Kim, J.-H., Kang, S.-O. & Hah, Y. C. ( 2000b; ). Saccharothrix violacea sp. nov., isolated from a gold mine cave, and Saccharothrix albidocapillata comb. nov. Int J Syst Evol Microbiol 50, 1315–1323.[CrossRef]
    [Google Scholar]
  14. MacFaddin, J. F. ( 1980; ). Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Minnikin, D. E. M. ( 1988; ). Isolation and purification of mycobacterial wall lipids. In Bacterial Cell Surface Techniques, pp. 125–135. Edited by I. C. Hancock & I. R. Poxton. Chichester: Wiley.
  17. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1977; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88, 200–204.
    [Google Scholar]
  18. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  19. Saddler, G. S., Tavecchia, P., Lociuro, S., Zanol, M., Colombo, E. & Selva, E. ( 1991; ). Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14, 185–191.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  22. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  24. Uchida, K. & Aida, K. ( 1984; ). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef]
    [Google Scholar]
  25. Yassin, A. F., Haggenei, B., Budzikiewicz, H. & Schaal, K. P. ( 1993; ). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast bombardment-mass spectrometry to the structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43, 414–420.[CrossRef]
    [Google Scholar]
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