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Abstract

Two novel Gram-positive, acidophilic bacterial strains were isolated from forest soil. According to their 16S rRNA gene sequences, these strains are related closely to each other and form a distinct cluster within the order . They show the typical features of filamentous actinomycetes, with branched vegetative hyphae and production of aerial hyphae. The distinct phylogenetic positions and the combination of chemotaxonomic characteristics of these strains justify the proposal of gen. nov. Both strains display 3-hydroxydiaminopimelic acid plus traces of -diaminopimelic acid, the phospholipids diphosphatidylglycerol, phosphatidylethanolamine, methylphosphatidylethanolamine and phosphatidylinositol, the predominant cellular fatty acids i-C, i-C and ai-C and the whole-cell sugars mannose and rhamnose. They differ in the fatty acid profiles, in the quantitative ratios of the major menaquinones MK-9(H), MK-9(H) and MK-9(H) and in the occurrence of additional whole-cell sugars (arabinose and xylose in strain GE134766 and galactose in strain GE134769). Differences in the phenotypic characteristics and in the 16S rRNA gene sequences suggest the description of two species, gen. nov., sp. nov. (the type species) and sp. nov., with the type strains GE134769 (=DSM 44927=NRRL B-24432) and GE134766 (=DSM 44926=NRRL B-24431), respectively. The DNA G+C contents of strains GE134769 and GE134766 are 70.8 and 69.2 mol%, respectively. Due to the large phylogenetic distance from known actinomycete genera, it is proposed to accommodate gen. nov. in fam. nov. In addition, subord. nov. is proposed to harbour fam. nov. and the newly proposed family , described in the accompanying paper.

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2006-08-01
2020-01-21
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Field emission scanning electron microscopy of GE134766 grown on HSA5 agar for 3 weeks at 28 ºC. Conic tufts of sporogenous aerial hyphae originated by the branching of single and short sporophorous hyphae can be seen.

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Field emission scanning electron microscopy of GE134769 grown on HSA5 agar for 3 weeks at 28 ºC. Tufts of sporogenous aerial hyphae undergoing division into squat to cylindrical arthrospores are shown. Branching of sporophorous hyphae giving origin to tufts appears very closed to the agar surface. Arrow indicates the rugosity of the surface of a not fragmented hypha.

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Neighbour-joining tree based on 1419 aligned positions within the 16S rRNA genes of GE134766 , GE134769 and related strains [ PDF] (100 KB)

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