1887

Abstract

The taxonomic position of two mycolic-acid-producing actinomycetes, isolates J81 and J82, which were recovered from activated sludge foam, was clarified. Comparative 16S rRNA gene sequence studies indicated that the organisms formed a distinct lineage within the 16S rRNA gene tree. The taxonomic integrity of this group was underpinned by a wealth of phenotypic data, notably characteristic rudimentary right-angled branching. In addition, isolate J81 contained the following: -diaminopimelic acid, arabinose and galactose; -glycolated muramic acid residues; a dihydrogenated menaquinone with eight isoprene units as the predominant isoprenologue; a fatty acid profile rich in oleic and palmitoleic acids and with relatively small proportions of myristic, stearic and tuberculostearic acids; mycolic acids with 44–52 carbons; and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides as major polar lipids. Strain J81 was found to have a chemotaxonomic profile that serves to distinguish it from representatives of all of the other taxa classified as belonging to the suborder . In the light of these data, it is proposed that the two isolates be classified in a novel monospecific genus. The name proposed for this taxon is gen. nov., sp. nov.; strain J81 (=DSM 44463=NRRL B-24424) is the type strain of .

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2006-04-01
2021-03-07
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