1887

Abstract

A polyphasic approach was used to characterize a bacterium, GPTSA 11, isolated from a warm spring located in a reserve forest in Assam, India. The cells are Gram-variable, strictly aerobic, sporulating motile rods. The major fatty acids of the strain are C anteiso (48·42 %), C iso (11·59 %), C 11 (6·16 %), C iso (6·03 %), C anteiso (5·68 %) and C 7 alcohol (5·01 %). The presence of the fatty acid C 7 alcohol distinguishes this strain from other closely related species of the genus . The strain contains MK-7 as the diagnostic menaquinone. The G+C content of the genomic DNA is 41·2 mol%. Analysis of the 16S rRNA gene sequence (1466 nt) revealed the presence of signature sequences PAEN 515F (5′-GAGTAACTGCTCTCGGAATGACGGTACTTGAGAAGAAAGCCCC-3′) and PAEN 862F (5′-TCGATACCCTTGGTGCCGAAGT-3′), which were found in the species of the genus surveyed by Shida . [ Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. (1997) . , 289–298]. The sequence shows closest similarity (95·85 %) to that of , followed by (94·34 %), (93·87 %), (93·80 %), (93·47 %) and (93·40 %). Biochemical, physiological, chemotaxonomic and phylogenetic analyses justify placement of the strain in the genus but not within any existing species. It should, therefore, be considered as representing a novel species, for which the name sp. nov. is proposed. The type strain is GPTSA 11 (=MTCC 6934=JCM 13186).

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2005-11-01
2020-01-22
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