Full text loading...
Abstract
A polyphasic approach was used to characterize a bacterium, GPTSA 11T, isolated from a warm spring located in a reserve forest in Assam, India. The cells are Gram-variable, strictly aerobic, sporulating motile rods. The major fatty acids of the strain are C15 : 0 anteiso (48·42 %), C16 : 0 iso (11·59 %), C16 : 1 ω11c (6·16 %), C15 : 0 iso (6·03 %), C17 : 0 anteiso (5·68 %) and C16 : 1 ω7c alcohol (5·01 %). The presence of the fatty acid C16 : 1 ω7c alcohol distinguishes this strain from other closely related species of the genus Paenibacillus. The strain contains MK-7 as the diagnostic menaquinone. The G+C content of the genomic DNA is 41·2 mol%. Analysis of the 16S rRNA gene sequence (1466 nt) revealed the presence of signature sequences PAEN 515F (5′-GAGTAACTGCTCTCGGAATGACGGTACTTGAGAAGAAAGCCCC-3′) and PAEN 862F (5′-TCGATACCCTTGGTGCCGAAGT-3′), which were found in the species of the genus Paenibacillus surveyed by Shida et al. [ Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. (1997) . Int J Syst Bacteriol 47, 289–298]. The sequence shows closest similarity (95·85 %) to that of Paenibacillus apiarius, followed by Paenibacillus alvei (94·34 %), Paenibacillus cineris (93·87 %), Paenibacillus favisporus (93·80 %), Paenibacillus chibensis (93·47 %) and Paenibacillus azoreducens (93·40 %). Biochemical, physiological, chemotaxonomic and phylogenetic analyses justify placement of the strain in the genus Paenibacillus but not within any existing species. It should, therefore, be considered as representing a novel species, for which the name Paenibacillus assamensis sp. nov. is proposed. The type strain is GPTSA 11T (=MTCC 6934T=JCM 13186T).
- Published Online: