Indigenous bacteria found in the sediment of the Emerald Basin (depth of 215 m, Atlantic Ocean) located offshore of Halifax Harbour (Nova Scotia, Canada) were previously found to be able to degrade the explosive compound hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). In the present study, a novel obligately respiratory, denitrifying and RDX-mineralizing bacterium, designated strain HAW-EB4, was isolated from the marine sediment. This bacterium utilized peptone, yeast extract, Casamino acids, esters (Tweens 20, 40 and 80), sugars (-acetyl--glucosamine, ribose), several C and C acids (acetate, pyruvate, lactate, propionate) and amino acids (serine, proline) as sole carbon and energy sources. Aerobically grown cells (in marine broth 2216 at 10 °C) contained C (6 %), iso-C (12 %), C (20 %), C 7 (37 %), C 7 (7 %) and C 3 (7 %) as major membrane fatty acids, and Q7 (28·1 %) and MK-7 (60·9 %) as dominant respiratory quinones, consistent with deep-sea species of . The novel bacterium had a DNA G+C content of 45 mol% and showed similarity to species in terms of 16S rRNA and gene sequences (93–99 and 67·3–88·4 % similarity, respectively), with being the most closely related species. Genomic DNA–DNA hybridization between strain HAW-EB4 and revealed a level of relatedness of 17·9 %, lower than the 70 % species cut-off value, indicating that strain HAW-EB4 (=NCIMB 14093=DSM 17350) is the type strain of a novel species of , for which the name sp. nov. is proposed.


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