1887

Abstract

Two Gram-negative, yellow-pigmented bacteria designated PSD1-4 and PHA3-4, isolated from two sand-dune plant species inhabiting coastal areas in Tae-an, Korea, were subjected to taxonomic investigation. 16S rRNA gene sequence analysis indicated that both isolates should be placed in the genus of the family . The phenotypic properties of the strains were also consistent with their classification into this genus. The levels of 16S rRNA gene sequence similarity between strain PSD1-4 and other species were 95·2–97·2 %; those between PHA3-4 and others were 93·7–97·8 %. The DNA–DNA relatedness data indicated that strains PSD1-4 and PHA3-4 were clearly different from the nearest species, and . The major fatty acids were 13-methyltetradecanoic acid (iso-C15 : 0), 3-hydroxy-15-methylhexadecanoic acid (iso-C17 : 0 3-OH) and omega-9--15-methylhexadecenoic acid (iso-C17 : 19) for both strains. On the basis of polyphasic taxonomic analysis results, it is evident that each of these strains represents a novel species of , for which the names sp. nov. (type strain PSD1-4=KCTC 12382=NBRC 100864) and sp. nov. (type strain PHA3-4=KCTC 12381=NBRC 100863) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63825-0
2006-02-01
2024-11-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/2/433.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63825-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Hugo C., Bruun B. 2001 The genus Chryseobacterium . In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , 3rd edn, release 3.7. Edited by Dworkin M. et al. New York: Springer; http://link.springer-ny.com/link/service/books/10125/
    [Google Scholar]
  2. de Beer H. D., Hugo C. J., Jooste P. J., Willems A., Vancanneyt M., Coenye T., Vandamme P. 2005; Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken processing plant. Int J Syst Evol Microbiol 55:2149–2153 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Han S. K., Nedashkovskaya O. I., Mikhailov V. V., Kim S. B., Bae K. S. 2003; Salinibacterium amurskyense gen. nov., sp. nov. a novel genus of the family Microbacteriaceae from the marine environment. Int J Syst Evol Microbiol 53:2061–2066 [CrossRef]
    [Google Scholar]
  6. Hugo C. J., Segers P., Hoste B., Vancanneyt M., Kersters K. 2003; Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53:771–777 [CrossRef]
    [Google Scholar]
  7. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
    [Google Scholar]
  8. Kim K. K., Bae H. S., Schumann P., Lee S. T. 2005; Chryseobacterium daecheongense sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 55:133–138 [CrossRef]
    [Google Scholar]
  9. Kim K. K., Kim M.-K., Lim J. H., Park H. Y., Lee S. T. 2005; Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol 55:1287–1293 [CrossRef]
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  11. Li Y., Kawamura K., Fujiwara N., Naka T., Liu H., Huang X., Kobayashi K., Ezaki T. 2003; Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir. Syst Appl Microbiol 26:523–528 [CrossRef]
    [Google Scholar]
  12. Lim Y. W., Baik K. S., Han S. K., Kim S. B., Bae K. S. 2003; Burkholderia sordidicola sp. nov., isolated from the white-rot fungus Phanerochaete sordida . Int J Syst Evol Microbiol 53:1631–1636 [CrossRef]
    [Google Scholar]
  13. McSpadden Gardener B. B., Weller D. M. 2001; Changes in populations of rhizosphere bacteria associated with take-all disease of wheat. Appl Environ Microbiol 67:4414–4425 [CrossRef]
    [Google Scholar]
  14. Mudarris M., Austin B., Segers P., Vancanneyt M., Hoste B., Bernardet J. F. 1994; Flavobacterium scophthalmum sp. nov., a pathogen of turbot ( Scophthalmus maximus L.). Int J Syst Bacteriol 44:447–453 [CrossRef]
    [Google Scholar]
  15. Park M. S., Jung S. R., Lee M. S., Kim K. O., Do J. O., Lee K. H., Kim S. B., Bae K. S. 2005; Isolation and characterization of bacteria associated with two sand dune plant species, Calystegia soldanella and Elymus mollis . J Microbiol 43:219–227
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI Inc;
    [Google Scholar]
  18. Shen F. T., Kämpfer P., Young C. C., Lai W. A., Arun A. B. 2005; Chryseobacterium taichungense sp. nov., isolated from contaminated soil. Int J Syst Evol Microbiol 55:1301–1304 [CrossRef]
    [Google Scholar]
  19. Shimomura K., Kaji S., Hiraishi A. 2005; Chryseobacterium shigense sp. nov., a yellow-pigmented, aerobic bacterium isolated from a lactic acid beverage. Int J Syst Evol Microbiol 55:1903–1906 [CrossRef]
    [Google Scholar]
  20. Vandamme P., Bernardet J.-F., Segers P., Kersters K., Holmes B. 1994; New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev.. Int J Syst Bacteriol 44827–831 [CrossRef]
    [Google Scholar]
  21. Young C. C., Kämpfer P., Shen F. T., Lai W. A., Arun A. B. 2005; Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55:423–426 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63825-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63825-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error