1887

Abstract

Two Gram-negative, yellow-pigmented bacteria designated PSD1-4 and PHA3-4, isolated from two sand-dune plant species inhabiting coastal areas in Tae-an, Korea, were subjected to taxonomic investigation. 16S rRNA gene sequence analysis indicated that both isolates should be placed in the genus of the family . The phenotypic properties of the strains were also consistent with their classification into this genus. The levels of 16S rRNA gene sequence similarity between strain PSD1-4 and other species were 95·2–97·2 %; those between PHA3-4 and others were 93·7–97·8 %. The DNA–DNA relatedness data indicated that strains PSD1-4 and PHA3-4 were clearly different from the nearest species, and . The major fatty acids were 13-methyltetradecanoic acid (iso-C15 : 0), 3-hydroxy-15-methylhexadecanoic acid (iso-C17 : 0 3-OH) and omega-9--15-methylhexadecenoic acid (iso-C17 : 19) for both strains. On the basis of polyphasic taxonomic analysis results, it is evident that each of these strains represents a novel species of , for which the names sp. nov. (type strain PSD1-4=KCTC 12382=NBRC 100864) and sp. nov. (type strain PHA3-4=KCTC 12381=NBRC 100863) are proposed.

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2006-02-01
2019-10-19
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References

  1. Bernardet, J.-F., Hugo, C. & Bruun, B. ( 2001; ). The genus Chryseobacterium. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community, 3rd edn, release 3.7. Edited by M. Dworkin et al. New York: Springer. http://link.springer-ny.com/link/service/books/10125/
  2. de Beer, H. D., Hugo, C. J., Jooste, P. J., Willems, A., Vancanneyt, M., Coenye, T. & Vandamme, P. ( 2005; ). Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken processing plant. Int J Syst Evol Microbiol 55, 2149–2153.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  5. Han, S. K., Nedashkovskaya, O. I., Mikhailov, V. V., Kim, S. B. & Bae, K. S. ( 2003; ). Salinibacterium amurskyense gen. nov., sp. nov., a novel genus of the family Microbacteriaceae from the marine environment. Int J Syst Evol Microbiol 53, 2061–2066.[CrossRef]
    [Google Scholar]
  6. Hugo, C. J., Segers, P., Hoste, B., Vancanneyt, M. & Kersters, K. ( 2003; ). Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53, 771–777.[CrossRef]
    [Google Scholar]
  7. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J. ( 2003; ). Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef]
    [Google Scholar]
  8. Kim, K. K., Bae, H. S., Schumann, P. & Lee, S. T. ( 2005; ). Chryseobacterium daecheongense sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 55, 133–138.[CrossRef]
    [Google Scholar]
  9. Kim, K. K., Kim, M.-K., Lim, J. H., Park, H. Y. & Lee, S. T. ( 2005; ). Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol 55, 1287–1293.[CrossRef]
    [Google Scholar]
  10. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  11. Li, Y., Kawamura, K., Fujiwara, N., Naka, T., Liu, H., Huang, X., Kobayashi, K. & Ezaki, T. ( 2003; ). Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir. Syst Appl Microbiol 26, 523–528.[CrossRef]
    [Google Scholar]
  12. Lim, Y. W., Baik, K. S., Han, S. K., Kim, S. B. & Bae, K. S. ( 2003; ). Burkholderia sordidicola sp. nov., isolated from the white-rot fungus Phanerochaete sordida. Int J Syst Evol Microbiol 53, 1631–1636.[CrossRef]
    [Google Scholar]
  13. McSpadden Gardener, B. B. & Weller, D. M. ( 2001; ). Changes in populations of rhizosphere bacteria associated with take-all disease of wheat. Appl Environ Microbiol 67, 4414–4425.[CrossRef]
    [Google Scholar]
  14. Mudarris, M., Austin, B., Segers, P., Vancanneyt, M., Hoste, B. & Bernardet, J. F. ( 1994; ). Flavobacterium scophthalmum sp. nov., a pathogen of turbot (Scophthalmus maximus L.). Int J Syst Bacteriol 44, 447–453.[CrossRef]
    [Google Scholar]
  15. Park, M. S., Jung, S. R., Lee, M. S., Kim, K. O., Do, J. O., Lee, K. H., Kim, S. B. & Bae, K. S. ( 2005; ). Isolation and characterization of bacteria associated with two sand dune plant species, Calystegia soldanella and Elymus mollis. J Microbiol 43, 219–227.
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Shen, F. T., Kämpfer, P., Young, C. C., Lai, W. A. & Arun, A. B. ( 2005; ). Chryseobacterium taichungense sp. nov., isolated from contaminated soil. Int J Syst Evol Microbiol 55, 1301–1304.[CrossRef]
    [Google Scholar]
  19. Shimomura, K., Kaji, S. & Hiraishi, A. ( 2005; ). Chryseobacterium shigense sp. nov., a yellow-pigmented, aerobic bacterium isolated from a lactic acid beverage. Int J Syst Evol Microbiol 55, 1903–1906.[CrossRef]
    [Google Scholar]
  20. Vandamme, P., Bernardet, J.-F., Segers, P., Kersters, K. & Holmes, B. ( 1994; ). New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44, 827–831.[CrossRef]
    [Google Scholar]
  21. Young, C. C., Kämpfer, P., Shen, F. T., Lai, W. A. & Arun, A. B. ( 2005; ). Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55, 423–426.[CrossRef]
    [Google Scholar]
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