1887

Abstract

Two Gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strains, DSW-1 and DSW-21, were isolated from sea water and subjected to a polyphasic taxonomic study. They grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. Strains DSW-1 and DSW-21 were characterized chemotaxonomically as containing MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH and iso-C as the major fatty acids. Their DNA G+C content was 38 mol%. Strains DSW-1 and DSW-21 exhibited four nucleotide differences in their 16S rRNA gene sequences and possessed a mean DNA–DNA relatedness level of 78 %. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains DSW-1 and DSW-21 formed a distinct lineage within the family . The 16S rRNA gene sequences of strains DSW-1 and DSW-21 had similarity levels of less than 92·2 % to the sequences of other members of the family . There were some differences in phenotypic properties between the two strains and species, the nearest phylogenetic neighbours. On the basis of phenotypic, phylogenetic and genetic data, strains DSW-1 (=KCTC 12391=DSM 17200) and DSW-21 were classified in a novel genus and species, gen. nov., sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63817-0
2005-11-01
2019-09-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/6/2323.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63817-0&mimeType=html&fmt=ahah

References

  1. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  2. Bowman, J. P. ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868.
    [Google Scholar]
  3. Bowman, J. P., McCammon, S. A., Brown, M. V., Nichols, D. S. & McMeekin, T. A. ( 1997; ). Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63, 3068–3078.
    [Google Scholar]
  4. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  5. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  7. Glöckner, F. O., Fuchs, B. M. & Amann, R. ( 1999; ). Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65, 3721–3726.
    [Google Scholar]
  8. Johansen, J. E., Nielsen, P. & Sjøholm, C. ( 1999; ). Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov. Int J Syst Bacteriol 49, 1231–1240.[CrossRef]
    [Google Scholar]
  9. Kirchman, D. L. ( 2002; ). The ecology of Cytophaga-Flavobacteria in aquatic environments. FEMS Microbiol Ecol 39, 91–100.
    [Google Scholar]
  10. Komagata, K. & Suzuki, K. ( 1987; ). Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–203.
    [Google Scholar]
  11. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  12. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  13. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  14. Nedashkovskaya, O. I., Suzuki, M., Lysenko, A. M., Snauwaert, C., Vancanneyt, M., Swings, J., Vysotskii, M. & Mikhailov, V. V. ( 2004; ). Cellulophaga pacifica sp. nov. Int J Syst Evol Microbiol 54, 609–613.[CrossRef]
    [Google Scholar]
  15. Nedashkovskaya, O. I., Kim, S. B., Han, S. K. & 9 other authors ( 2005a; ). Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 55, 49–55.[CrossRef]
    [Google Scholar]
  16. Nedashkovskaya, O. I., Kim, S. B., Lee, K. H., Bae, K. S., Frolova, G. M., Mikhailov, V. V. & Kim, I. S. ( 2005b; ). Pibocella ponti gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi. Int J Syst Evol Microbiol 55, 177–181.[CrossRef]
    [Google Scholar]
  17. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Frolova, G. M., Mikhailov, V. V., Lee, K. H. & Bae, K. S. ( 2005c; ). Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov. Int J Syst Evol Microbiol 55, 225–229.[CrossRef]
    [Google Scholar]
  18. Nedashkovskaya, O. I., Kim, S. B., Lee, D. H., Lysenko, A. M., Shevchenko, L. S., Frolova, G. M., Mikhailov, V. V., Lee, K. H. & Bae, K. S. ( 2005d; ). Roseivirga ehrenbergii gen. nov., sp. nov., a novel marine bacterium of the phylum ‘Bacteroidetes’, isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 55, 231–234.[CrossRef]
    [Google Scholar]
  19. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Frolova, G. M., Mikhailov, V. V. & Bae, K. S. ( 2005e; ). Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea. Int J Syst Evol Microbiol 55, 375–378.[CrossRef]
    [Google Scholar]
  20. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Frolova, G. M., Mikhailov, V. V., Bae, K. S., Lee, D. H. & Kim, I. S. ( 2005f; ). Gramella echinicola gen. nov., sp. nov., a novel halophilic bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 55, 391–394.[CrossRef]
    [Google Scholar]
  21. Reichenbach, H. ( 1992; ). The order Cytophagales. In the Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  22. Sasser, M. ( 1990; ). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. Newark, DE: MIDI.
  23. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S. ( 2001; ). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51, 1639–1652.[CrossRef]
    [Google Scholar]
  24. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  25. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  26. Yi, H., Yoon, H. I. & Chun, J. ( 2005; ). Sejongia antarctica gen. nov., sp. nov. and Sejongia jeonii sp. nov., isolated from the Antarctic. Int J Syst Evol Microbiol 55, 409–416.[CrossRef]
    [Google Scholar]
  27. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  28. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  29. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Kang, S.-J., Lee, C.-H. & Oh, T.-K. ( 2005; ). Marinicola seohaensis gen. nov., sp. nov., isolated from sea water of the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 859–863.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63817-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63817-0
Loading

Data & Media loading...

vol. , part 6, pp. 2323-2328

Cellular fatty acid composition of gen. nov., sp. nov., and species. [PDF](25 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error