1887

Abstract

Two Gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strains, DSW-1 and DSW-21, were isolated from sea water and subjected to a polyphasic taxonomic study. They grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. Strains DSW-1 and DSW-21 were characterized chemotaxonomically as containing MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH and iso-C as the major fatty acids. Their DNA G+C content was 38 mol%. Strains DSW-1 and DSW-21 exhibited four nucleotide differences in their 16S rRNA gene sequences and possessed a mean DNA–DNA relatedness level of 78 %. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains DSW-1 and DSW-21 formed a distinct lineage within the family . The 16S rRNA gene sequences of strains DSW-1 and DSW-21 had similarity levels of less than 92·2 % to the sequences of other members of the family . There were some differences in phenotypic properties between the two strains and species, the nearest phylogenetic neighbours. On the basis of phenotypic, phylogenetic and genetic data, strains DSW-1 (=KCTC 12391=DSM 17200) and DSW-21 were classified in a novel genus and species, gen. nov., sp. nov.

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2005-11-01
2021-10-20
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