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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 55, Issue 6

sp. nov., isolated from a marine solar saltern in Korea Free

Abstract

A Gram-positive or Gram-variable, motile, endospore-forming, halophilic bacterial strain, MSS-402, was isolated from a marine solar saltern in Korea, and subjected to a polyphasic taxonomic study. Some cells of strain MSS-402 were long filamentous rods. The isolate grew optimally at 37 °C and in the presence of 3–5 % (w/v) NaCl. Strain MSS-402 had cell-wall peptidoglycan based on -orn–-Asp, MK-7 as the predominant menaquinone and anteiso-C, anteiso-C and iso-C as major fatty acids. The DNA G+C content was 42·9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MSS-402 falls within the evolutionary radiation of species of the genus . Levels of 16S rRNA gene sequence similarity between strain MSS-402 and the type strains of recognized species ranged from 98·0 % (with ) to 99·2 % (with and ). Levels of DNA–DNA binding indicated that strain MSS-402 represents a genomic species that is distinct from recognized species. Strain MSS-402 was differentiated from species by means of several phenotypic characteristics. On the basis of its phenotypic properties and phylogenetic and genetic distinctiveness, strain MSS-402 (=KCTC 3957=DSM 17110) should be classified as the type strain of a novel species, for which the name sp. nov. is proposed.

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2005-11-01
2025-01-19
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References

  1. Amoozegar M. A., Malekzadeh F., Malik K. A., Schumann P., Spröer C. 2003; Halobacillus karajensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 53:1059–1063 [CrossRef]
     [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov. sp. nov. a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 511997–2006 [CrossRef]
     [Google Scholar]
  3. Claus D., Fahmy F., Rolf H. J., Tosunoglu N. 1983; Sporosarcina halophila sp. nov., an obligate, slightly halophilic bacterium from salt marsh soils. Syst Appl Microbiol 4:496–506 [CrossRef]
     [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  6. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
     [Google Scholar]
  7. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
     [Google Scholar]
  8. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
     [Google Scholar]
  9. Lim J.-M., Jeon C. O., Song S. M., Kim C.-J. 2005; Pontibacillus chungwhensis gen. nov., sp. nov. a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55:165–170 [CrossRef]
     [Google Scholar]
  10. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI;
     [Google Scholar]
  11. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
     [Google Scholar]
  12. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [CrossRef]
     [Google Scholar]
  13. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K.-H. 1996; Halobacillus gen. nov., with description of Halobacillus litoralis sp.nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
     [Google Scholar]
  14. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  15. Wainø M., Tindall B. J., Schumann P., Ingvorsen K. 1999; Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49:821–831 [CrossRef]
     [Google Scholar]
  16. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  17. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
     [Google Scholar]
  18. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
     [Google Scholar]
  19. Yoon J.-H., Weiss N., Lee K.-C., Lee I.-S., Kang K. H., Park Y.-H. 2001; Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov. Int J Syst Evol Microbiol 51:2087–2093 [CrossRef]
     [Google Scholar]
  20. Yoon J.-H., Kang K. H., Park Y.-H. 2002; Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 52:2043–2048 [CrossRef]
     [Google Scholar]
  21. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53:687–693 [CrossRef]
     [Google Scholar]
  22. Yoon J.-H., Kang K. H., Oh T.-K., Park Y.-H. 2004; Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8:23–28 [CrossRef]
     [Google Scholar]
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Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea
Int J Syst Evol Microbiol 55, 2413 (2005); https://doi.org/10.1099/ijs.0.63801-0
/content/journal/ijsem/10.1099/ijs.0.63801-0
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