1887

Abstract

A novel Gram-positive bacterial strain, designated KV-614, was isolated from a soil sample using an agar medium supplemented with superoxide dismutase. Based on 16S rRNA gene sequence analysis, it was found that the strain represented a novel deep-rooting lineage within the class and clustered with yet-uncultivated bacteria from terrestrial environments and some unidentified strains isolated by unique methods. The most closely related established genus was (92·4 % sequence similarity to DSM 14684). Cells of strain KV-614 were rod-shaped and motile with long flagella. The strain was catalase-positive, oxidase-negative and grew under aerobic conditions. The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid and alanine and glutamic acid. The peptidoglycan acyl type was acetyl. The only detected isoprenoid quinone was demethylmenaquinone with seven isoprene units (DMK-7). Mycolic acids were not detected. The predominant cellular fatty acid was 9-octadecenoic acid (C18 : 19). Minor components were 12-methyl tetradecanoic acid (anteiso-C15 : 0) and 14-methyl hexadecanoic acid (anteiso-C17 : 0). The DNA G+C content was 72 mol%. On the basis of phenotypic and genotypic characteristics, it is proposed that strain KV-614 represents a new genus and a novel species, gen. nov., sp. nov., in the class . The type strain is KV-614 (=NRRL B-24346=JCM 12834=NBRC 100761). The creation of the family fam. nov. is proposed to encompass the genus gen. nov.

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2006-02-01
2019-12-13
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