1887

Abstract

Two Gram-negative, rod-shaped bacterial strains, KSL-102 and KSL-110, were isolated from an alkaline soil in Korea, and their taxonomic positions were investigated by use of a polyphasic study. The two strains grew optimally at pH 7·0–8·0 and 30 °C without NaCl. They contained Q-10 as the predominant ubiquinone. The major fatty acids were C 7 and C on trypticase soy agar, but 11-methyl C 7 was also a major component when the two strains were cultivated on LMG medium no. 221. Their DNA G+C contents were 68·4–68·7 mol%. Strains KSL-102 and KSL-110 exhibited three nucleotide differences in their 16S rRNA gene sequences and a mean DNA–DNA relatedness value of 85 %. Phylogenetic trees based on 16S rRNA gene sequences showed that the two strains fell within the evolutionary radiation encompassed by the genus . Levels of 16S rRNA gene sequence similarity between the two strains and the type strains of recognized species ranged from 96·3 to 98·4 %. DNA–DNA relatedness levels between the two strains and recognized species were 8–21 %. On the basis of phenotypic, phylogenetic and genetic data, strains KSL-102 and KSL-110 were classified in the genus as members of a novel species, for which the name sp. nov. is proposed. The type strain is KSL-102 (=KCTC 12380=DSM 17033).

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2006-03-01
2019-10-23
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Supplements

vol. , part 3, pp. 613 - 617

Biolog assimilation data for strains KSL-102 and KSL-110 and some related species.

Cellular fatty acid compositions (%) of strains KSL-102 and KSL-110 and the type strains of some species cultivated on TSA and/or LMG medium no. 221.

[PDF file of Supplementary Tables S1 and S2](36 KB)



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