1887

Abstract

Two novel strains, Z-1 and Z-8, were isolated from a distilled-spirit-fermenting cellar in Hebei Province, China. The cells were Gram-positive, non-spore-forming, non-motile cocci, occurring in pairs or tetrads. The strains were facultatively anaerobic and produced 50 % (−)--/50 % (+)--lactic acid exclusively from glucose fermentation, but did not produce catalase. Both strains fermented rhamnose, ribose, xylose, lactose, maltose, trehalose and sucrose, and tolerated 10 % ethanol. The mean G+C content of the two strains was 38±1 mol% (37 mol% for the type strain, Z-8). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Z-8 clustered within the group, with 98·3–98·5 % 16S rRNA gene similarity. DNA–DNA relatedness between strain Z-8 and LMG 11484, LMG 11409 and LMG 11486 was 19±4, 35±5 and 31±5 %, respectively. Therefore, based on the distinctive phenotypic characteristics, and genotypic and phylogenetic analyses, a novel species, sp. nov., is proposed. The type strain is Z-8 (=AS 1.3787=LMG 22956).

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2005-09-01
2019-12-15
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