1887

Abstract

A Gram-positive, aerobic, long-rod-shaped, non-spore-forming bacterium (strain PPLB) was isolated from soil mixed with Iberian pig hair. This actinomycete showed keratinase activity when chicken feathers were added to the culture medium. Strain PPLB was oxidase-negative and catalase-positive and produced lipase and esterase lipase. This actinomycete grew at 40 °C on nutrient agar and in the same medium containing 5 % (w/v) NaCl. Growth was observed with many different carbohydrates as the sole carbon source. On the basis of 16S rRNA gene sequence similarity, strain PPLB was shown to belong to the genus of the family . Strain PPLB showed 98·8 % 16S rRNA gene sequence similarity to . Chemotaxonomic data, such as the main ubiquinone (MK-8), the main polar lipids (phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol) and the main fatty acids (i-C, ai-C, i-C and ai-C) supported the affiliation of strain PPLB to the genus . The G+C content of the DNA was 71 mol%. The results of DNA–DNA hybridization (36·6 % relatedness between and strain PPLB) and physiological and biochemical tests suggested that strain PPLB belongs to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PPLB (=CECT 3379=LMG 22921).

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2005-11-01
2020-01-22
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