DSM 43387, a clinical isolate from submandibular mycetoma tissue, is one of three plasmid-bearing strains of the genus . It degrades aromatic compounds such as -hydroxybenzoate, but does not produce any antibiotics, in contrast to NBRC 12806. Phylogenetic analysis based on a complete 16S rRNA gene sequence placed the strain in the clade of IMSNU 22139, distant from the clade of NBRC 12806. The strain showed low DNA–DNA hybridization values of 24·1 and 45·7 % with NBRC 12806 and DSM 44639 (=IMSNU 22139), respectively. It could also be readily distinguished from NBRC 12806 and all species with validly published names classified in the genus , by using a combination of chemical and physiological markers such as utilization of lactose, degradation of xanthine, hypoxanthine, gelatin and casein and hydrolysis of Tween 80, indicating that it represents a novel species. Strain DSM 43387 could also be differentiated from NBRC 12806 and its nearest neighbour IMSNU 22139 on the basis of fatty acid and phospholipid profiles. Based on genotypic and phenotypic differences, the name sp. nov. is proposed for strain DSM 43387 that was previously classified as . The type strain is AK 16/65 (=DSM 43387=ATCC 55165=IMRU 1389).


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  1. Arden-Jones, M. P., McCarthy, A. J. & Cross, T.(1979). Taxonomic and serologic studies on Micropolyspora faeni and Micropolyspora strains from soil bearing the specific epithet rectivirgula. J Gen Microbiol 115, 343–354.[CrossRef] [Google Scholar]
  2. Bala, S., Khanna, R., Dadhwal, M., Prabagaran, S. R., Shivaji, S., Cullum, J. & Lal, R.(2004). Reclassification of Amycolatopsis mediterranei DSM 46095 as Amycolatopsis rifamycinica sp. nov. Int J Syst Evol Microbiol 54, 1145–1149.[CrossRef] [Google Scholar]
  3. Christensen, W. B.(1946). Urea decomposition as a means of differentiating Proteus and para-colon cultures from each other and from Salmonella and Shigella types. J Bacteriol 52, 461–466. [Google Scholar]
  4. Collins, C. H., Lyne, P. M. & Grange, J. M.(1989).Microbiological Methods, 6th edn. London: Butterworth.
  5. Dhingra, G., Kumari, R., Bala, S., Majumdar, S., Malhotra, S., Sharma, P., Lal, S., Cullum, J. & Lal, R.(2003). Development of cloning vectors and transformation methods for Amycolatopsis. J Ind Microbiol Biotechnol 30, 195–204.[CrossRef] [Google Scholar]
  6. Embley, T. M., Smida, J. & Stackebrandt, E.(1988). The phylogeny of mycolate-less wall chemotype IV actinomycetes and the description of Pseudonocardiaceae fam. nov. Syst Appl Microbiol 11, 44–52.[CrossRef] [Google Scholar]
  7. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle. USA.
  9. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  10. Gordon, R. E., Mishra, S. K. & Barnett, D. A.(1978). Some bits and pieces of the genus Nocardia: N. carnea, N. vaccinii, N. transvalensis, N. orientalis and N. aerocolonigenes. J Gen Microbiol 109, 69–78.[CrossRef] [Google Scholar]
  11. Grund, E., Knorr, C. & Eichenlaub, R.(1990). Catabolism of benzoate and monohydroxylated benzoates by Amycolatopsis and Streptomyces spp. Appl Environ Microbiol 56, 1459–1464. [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  13. Kieser, T., Bibb, M. J., Buttner, M. J., Chater, K. F. & Hopwood, D. A.(2000).Practical Streptomyces Genetics. Norwich: John Innes Foundation.
  14. Lal, R., Lal, S., Grund, E. & Eichenlaub, R.(1991). Construction of a hybrid plasmid capable of replication in Amycolatopsis mediterranei. Appl Environ Microbiol 57, 665–671. [Google Scholar]
  15. Lechevalier, M. P. & Lechevalier, H.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  16. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef] [Google Scholar]
  17. Lechevalier, M. P., Prauser, H., Labeda, D. P. & Ruan, J.-S.(1986). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36, 29–37.[CrossRef] [Google Scholar]
  18. Lee, S. D. & Hah, Y. C.(2001).Amycolatopsis albidoflavus sp. nov. Int J Syst Evol Microbiol 51, 645–650. [Google Scholar]
  19. McCarthy, A. J. & Cross, T.(1984). A taxonomic study of Thermomonospora and other monosporic actinomycetes. J Gen Microbiol 130, 5–25. [Google Scholar]
  20. Mishra, S. K., Gordon, R. E. & Barnett, D. A.(1980). Identification of nocardiae and streptomycetes of medical importance. J Clin Microbiol 11, 728–736. [Google Scholar]
  21. Reddy, G. S. N., Aggarwal, R. K., Matsumoto, G. I. & Shivaji, S.(2000).Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50, 1553–1561.[CrossRef] [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  24. Scharfen, J.(1971). Trutonov 139-66. An unusual actinomycetes combining the contradictory properties of the genera Nocardia and Actinomyces – the causative agent of submandibular mycetoma. I. Introduction. J Hyg Epidemiol Microbiol Immunol 15, 43–51. [Google Scholar]
  25. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  26. Takahashi, Y.(2001). Family Pseudonocardiaceae. In Identification Manual of Actinomycetes, pp. 227–239. Edited by The Society for Actinomycetes, Japan. Tokyo: The Business Center for Academic Societies.
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  28. Tourova, T. P. & Antonov, A. S.(1987). Identification of microorganisms by rapid DNA–DNA hybridization. Methods Microbiol 19, 333–355. [Google Scholar]
  29. Warwick, S., Bowen, T., McVeigh, H. & Embley, T. M.(1994). A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia. Int J Syst Bacteriol 44, 293–299.[CrossRef] [Google Scholar]
  30. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  31. Yassin, A. F., Haggenei, B., Budzikiewicz, H. & Schaal, K. P.(1993). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast atom bombardment-mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43, 414–420.[CrossRef] [Google Scholar]

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