Three xylan-degrading actinobacterial strains were isolated from different sampling sites in the Roman catacombs of Domitilla and San Callisto. The organisms showed morphological and chemotaxonomic properties such as peptidoglycan type A4, -lys–-thr–-Glu; whole-cell sugars (glucose, mannose and galactose); octa-, hexa- and tetrahydrogenated menaquinones with nine isoprene units; phosphatidylglycerol and diphosphatidylglycerol as the major phospholipids; anteiso-C, iso-C and iso-C as the predominant fatty acids; and a DNA G+C content of 72 mol%. These features are consistent with affiliation of these isolates to the genus . The three isolates shared a 16S rRNA gene similarity of 99·9 % and were most closely related to DSM 15700 (97·9 % sequence similarity). The low level of DNA–DNA relatedness (about 14 %) and the differences in phenotypic characteristics between the novel strains and DSM 15700 justify the proposal of a novel species of the genus , sp. nov., with CD12E2-27 (=HKI 0369=DSM 17134=NCIMB 14061=VTT E-032285) as the type strain.


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  1. Becker, B., Lechevalier, M. P. & Lechevalier, H. A.(1965). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13, 236–243. [Google Scholar]
  2. Bruce, J.(1996). Automated system rapidly identifies and characterizes microorganisms in food. Food Technol 50, 77–81. [Google Scholar]
  3. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M.(1977). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef] [Google Scholar]
  4. Cazemier, A. E., Verdoes, J. C., Reubsaet, F. A. G., Hackstein, J. H. P., van der Drift, C. & Op den Camp, H. J. M.(2003).Promicromonospora pachnodae sp. nov., a member of the (hemi)cellulolytic hindgut flora of larvae of the scarab beetle Pachnoda marginata. Antonie Van Leeuwenhoek 83, 135–148.[CrossRef] [Google Scholar]
  5. Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef] [Google Scholar]
  6. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. Cambridge: Cambridge University Press.
  7. Cui, X., Schumann, P., Stackebrandt, E., Kroppenstedt, R. M., Pukall, R., Xu, L., Rohde, M. & Jiang, C.(2004).Myceligenerans xiligouense gen. nov., sp. nov., a novel hyphae-forming member of the family Promicromonosporaceae. Int J Syst Evol Microbiol 54, 1287–1293.[CrossRef] [Google Scholar]
  8. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  9. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5.1. Distributed by the author, Department of Genome Sciences, University of Washington, Seattle, USA.
  10. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  11. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  12. Groth, I., Schumann, P., Schütze, B., Gonzalez, J. M., Laiz, L., Saiz-Jimenez, C. & Stackebrandt, E.(2005).Isoptericola hypogeus sp. nov., isolated from the Roman catacomb of Domitilla. Int J Syst Evol Microbiol 55, 1715–1719.[CrossRef] [Google Scholar]
  13. Hayakawa, M. & Nonomura, H.(1987). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65, 501–509.[CrossRef] [Google Scholar]
  14. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  15. Krasil'nikov, N. A., Kalakoutskii, L. V. & Kirillova, N. F.(1961). New genus of the Actinomycetales: Promicromonospora gen. nov. Izv Akad Nauk SSSR Ser Biol 1, 107–112 (in Russian). [Google Scholar]
  16. Lanyi, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  17. MacKenzie, S. L.(1987). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160. [Google Scholar]
  18. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  19. Minnikin, D. E., Alshamaony, L. & Goodfellow, M.(1975). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef] [Google Scholar]
  20. Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef] [Google Scholar]
  21. Prauser, H. & Falta, R.(1968). Phage sensitivity, cell wall composition and taxonomy of actinomycetes. Z Allg Mikrobiol 8, 39–46 (in German).[CrossRef] [Google Scholar]
  22. Rivas, R., Sánchez, M., Trujillo, M. E., Zurdo-Piñeiro, J. L., Mateos, P. F., Martínez-Molina, E. & Velázquez, E.(2003).Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree (Ulmus nigra). Int J Syst Evol Microbiol 53, 99–103.[CrossRef] [Google Scholar]
  23. Rivas, R., Trujillo, M. E., Schumann, P., Kroppenstedt, R. M., Sánchez, M., Mateos, P. F., Martínez-Molina, E. & Velázquez, E.(2004).Xylanibacterium ulmi gen. nov., sp. nov., a novel xylanolytic member of the family Promicromonosporaceae. Int J Syst Evol Microbiol 54, 557–561.[CrossRef] [Google Scholar]
  24. Saddler, G. S., Tavecchia, P., Lociuro, S., Zanol, M., Colombo, L. & Selva, E.(1991). Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14, 185–191.[CrossRef] [Google Scholar]
  25. Schleifer, K. H.(1985). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156. [Google Scholar]
  26. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. Methods Gen Mol Bacteriol 25, 607–654. [Google Scholar]
  28. Stackebrandt, E. & Schumann, P.(2004). Reclassification of Promicromonospora pachnodae Cazemier et al. 2004 as Xylanimicrobium pachnodae gen. nov., comb. nov. Int J Syst Evol Microbiol 54, 1383–1386.[CrossRef] [Google Scholar]
  29. Stackebrandt, E., Schumann, P. & Cui, X.-L.(2004). Reclassification of Cellulosimicrobium variabile Bakalidou et al. 2002 as Isoptericola variabilis gen. nov. comb. nov. Int J Syst Evol Microbiol 54, 685–688.[CrossRef] [Google Scholar]
  30. Uchida, K. & Aida, K.(1984). An improved method for the glycolate test for simple identification of the acyl type of bacteria cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef] [Google Scholar]
  31. Yokota, A., Takeuchi, M., Sakane, T. & Weiss, N.(1993). Proposal of six new species of the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov. Int J Syst Bacteriol 43, 555–564.[CrossRef] [Google Scholar]

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vol. , part 1, pp. 283-287

Physiological test results for the strains in the study. [PDF](43 KB)


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