1887

Abstract

Three xylan-degrading actinobacterial strains were isolated from different sampling sites in the Roman catacombs of Domitilla and San Callisto. The organisms showed morphological and chemotaxonomic properties such as peptidoglycan type A4, -lys–-thr–-Glu; whole-cell sugars (glucose, mannose and galactose); octa-, hexa- and tetrahydrogenated menaquinones with nine isoprene units; phosphatidylglycerol and diphosphatidylglycerol as the major phospholipids; anteiso-C, iso-C and iso-C as the predominant fatty acids; and a DNA G+C content of 72 mol%. These features are consistent with affiliation of these isolates to the genus . The three isolates shared a 16S rRNA gene similarity of 99·9 % and were most closely related to DSM 15700 (97·9 % sequence similarity). The low level of DNA–DNA relatedness (about 14 %) and the differences in phenotypic characteristics between the novel strains and DSM 15700 justify the proposal of a novel species of the genus , sp. nov., with CD12E2-27 (=HKI 0369=DSM 17134=NCIMB 14061=VTT E-032285) as the type strain.

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2006-01-01
2019-10-20
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vol. , part 1, pp. 283-287

Physiological test results for the strains in the study. [PDF](43 KB)



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