1887

Abstract

The aim of this study was to clarify the taxonomic position of the nitrogen-fixing and hydrogen-oxidizing bacteria strains IAM 12599, IAM 12664 and IAM 12665 and IAM 14368. It was found that the type strain of , IAM 12599, showed 99·9 and 96·1 % 16S rRNA gene sequence similarity to strains IAM 12665 and IAM 12664, respectively. A comparison using DNA–DNA hybridization suggested that strains IAM 12599 and IAM 12665 belong to a single species (89·7 %) and that strain IAM 12664 (35·1 %) forms a separate species. The phenotypic characteristics also support the conclusion that these bacteria should be identified as two species of a new genus: gen. nov., comb. nov. (type strain IAM 12599=DSM 1122=LMG 3321=ATCC 29712; reference strain IAM 12665=DSM 1123=LMG 3325=ATCC 29714) and sp. nov. (type strain IAM 12664=DSM 1124=LMG 3324=ATCC 29713). IAM 14368 was found to be closely related to the phototrophic bacterium , with 96·8 % 16S rRNA gene sequence similarity, but the two bacteria are quite different with respect to their metabolism and some significant phenotypic characteristics, suggesting that they cannot be included in a single genus. Further studies on their gene sequences, G+C content of the DNA and cellular fatty acid composition confirm that should be reclassified: the name gen. nov., comb. nov. is proposed, with the type strain IAM 14368 (=LMG 2256=ATCC 15946).

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2005-11-01
2020-08-06
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