1887

Abstract

A Gram-negative, rod-shaped, non-spore-forming bacteria was isolated from a nitrifying inoculum. On the basis of 16S rRNA gene sequence similarity, this strain, designated LMG 22951, was shown to belong to the ‘’ and to be related to (97·4 %) and (97·0 %). The results of DNA–DNA hybridization, analysis of fatty acid composition, SDS-PAGE, physiological and biochemical tests allowed genotypic and phenotypic differentiation of LMG 22951 from the two recognized species. LMG 22951 therefore represents a novel species within this genus, for which the name is proposed. The type strain is LMG 22951 (=DSM 17137).

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2005-09-01
2021-10-16
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References

  1. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 45:493–496
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Foster J. W. 1944; Microbiological aspects of riboflavin Introduction I, II. Bacterial oxidation of riboflavin to lumichrome. J Bacteriol 4727–41
    [Google Scholar]
  4. Grommen R., Van Hauteghem I., Van Wambeke M., Verstraete W. 2002; An improved nitrifying enrichment to remove ammonium and nitrite from freshwater aquaria systems. Aquaculture 211:115–124 [CrossRef]
    [Google Scholar]
  5. Heyndrickx M., Vauterin L., Vandamme P., Kersters K., De Vos P. 1996; Applicability of combined amplified ribosomal DNA restriction analysis (ARDRA) patterns in bacterial phylogeny and taxonomy. J Microbiol Methods 26:247–259 [CrossRef]
    [Google Scholar]
  6. Heyrman J., Swings J. 2001; 16S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the Servilia tomb (necropolis of Carmona, Seville, Spain. Syst Appl Microbiol 24:417–422 [CrossRef]
    [Google Scholar]
  7. Logan N. A., Lebbe L., Hoste B. 7 other authors 2000; Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50:1741–1753
    [Google Scholar]
  8. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  9. Nakagawa Y., Sakane T., Yokota A. 1996; Transfer of “ Pseudomonas riboflavina ” (Foster 1944), a Gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev. Int J Syst Bacteriol 4616–22 [CrossRef]
    [Google Scholar]
  10. NCCLS 1993 Performance standards for antimicrobial disk susceptibility tests , 5th edn. Approved standard M2-A5 Vilanove, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  11. Pitcher D. G., Saunders L. A., Owen N. A. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  12. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  13. Rivas R., Velázquez E., Willems A., Vizcaíno N., Subba-Rao N. S., Mateos P. F., Gillis M., Dazzo F. B., Martínez-Molina E. 2002; A new species of Devosia that forms a unique nitrogen-fixing root-nodule symbiosis with the aquatic legume Neptunia natans (L.f.) Druce. Appl Environ Microbiol 68:5217–5222 [CrossRef]
    [Google Scholar]
  14. Rivas R., Willems A., Subba-Rao N. S., Mateos P. F., Dazzo F. B., Kroppenstedt R. M., Martínez-Molina E., Gillis M., Velázquez E. 2003; Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans , an aquatic legume from India. Syst Appl Microbiol 26:47–53 [CrossRef]
    [Google Scholar]
  15. Smibert R. M., Krieg N. R. 1994 In Methods for General and Molecular Bacteriology , p. 649 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43:159–271 [CrossRef]
    [Google Scholar]
  17. Vandamme P., Vancanneyt M., Pot B. 10 other authors 1992; Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42:344–356 [CrossRef]
    [Google Scholar]
  18. Vannini C., Rosati G., Verni F., Petroni G. 2004; Identification of the bacterial endosymbionts of the marine ciliate Euplotes magnicirratus (Ciliophora, Hypotrichia) and proposal of ‘ Candidatus Devosia euplotis’. Int J Syst Evol Microbiol 54:1151–1156 [CrossRef]
    [Google Scholar]
  19. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M. 2001; DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51:1315–1322
    [Google Scholar]
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