1887

Abstract

A Gram-negative, motile, denitrifying bacterium (strain AcBE2-1) was isolated from activated sludge of a municipal wastewater treatment plant using 17-oestradiol (E2) as sole source of carbon and energy. Cells were curved rods, 0.4–0.8×0.8–2.0 μm in size, non-fermentative, non-spore-forming, oxidase-positive and catalase-negative. E2 was oxidized completely to carbon dioxide and water by reduction of nitrate to a mixture of dinitrogen monoxide and dinitrogen, with the intermediate accumulation of nitrite. Electron recoveries were between 90 and 100 %, taking assimilated E2 into account. With nitrate as the electron acceptor, the bacterium also grew on fatty acids (C to C), isobutyrate, crotonate, -lactate, pyruvate, fumarate and succinate. Phylogenetic analysis of its 16S rRNA gene sequence revealed that strain AcBE2-1 represents a separate line of descent within the family (). The closest relatives are the cholesterol-degrading, denitrifying bacteria DSM 13999 and strain 72Chol (=DSM 12783), with <93.9 % sequence similarity. The G+C content of the DNA was 61.4 mol%. Detection of a quinone system with ubiquinone Q-8 as the predominant compound and a fatty acid profile that included high concentrations of C 7/iso-C 2-OH and C, in addition to C 7 and small amounts of C 3-OH, supported the results of the phylogenetic analysis. On the basis of 16S rRNA gene sequence data in combination with chemotaxonomic and physiological data, strain AcBE2-1 (=DSM 16959=JCM 12830) is placed in a new genus gen. nov. as the type strain of the type species gen. nov., sp. nov.

Keyword(s): E2, 17β-oestradiol
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2006-07-01
2021-10-19
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