1887

Abstract

Genetic relationships among bacterial strains belonging to the genus were inferred from 16S rRNA, and gene sequences. Twenty-eight type or collection strains of the recognized species or subspecies and 33 strains isolated from human and animal specimens as well as from environmental samples were included in the study. As reported previously, the 16S rRNA gene sequence is highly conserved within the genus , having only limited resolution for this very tight group of species. Analysis of a 1.1 kb sequence confirmed that this gene has high resolving power, with maximal interspecies divergence of 15.2 %. Similar results were obtained by sequencing only 517 bp of the gene, which showed maximal interspecies divergence of 13 %. The topologies of the - and -derived trees were similar. The results confirm the close relationship of species within the genus and show that a phylogenetic approach including several genes is suitable for improving the complicated taxonomy of the genus.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63650-0
2006-12-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/12/2743.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63650-0&mimeType=html&fmt=ahah

References

  1. Amann, R. I., Ludwig, W. & Schleifer, K. H. ( 1995; ). Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59, 143–169.
    [Google Scholar]
  2. Crivelli, C., Demarta, A. & Peduzzi, R. ( 2001; ). Intestinal secretory immunoglobulin A (sIgA) response to Aeromonas exoproteins in patients with naturally acquired Aeromonas diarrhea. FEMS Immunol Med Microbiol 30, 31–35.[CrossRef]
    [Google Scholar]
  3. Demarta, A., Tonolla, M., Caminada, A.-P., Ruggeri, N. & Peduzzi, R. ( 1999; ). Signature region within the 16S rRNA sequences of Aeromonas popoffii. FEMS Microbiol Lett 172, 239–246.[CrossRef]
    [Google Scholar]
  4. Demarta, A., De Respinis, S., Dolina, M. & Peduzzi, R. ( 2004a; ). Molecular typing of Yersinia frederiksenii strains by means of 16S rRNA and gyrB genes sequence analyses. FEMS Microbiol Lett 238, 423–428.
    [Google Scholar]
  5. Demarta, A., Huys, G., Tonolla, M., Swings, J. & Peduzzi, R. ( 2004b; ). Polyphasic taxonomic study of “Aeromonas eucrenophila-like” isolates from clinical and environmental sources. Syst Appl Microbiol 27, 343–349.[CrossRef]
    [Google Scholar]
  6. Demarta, A., Tonolla, M., Caminada, A. P., Beretta, M. & Peduzzi, R. ( 2000; ). Epidemiological relationships between Aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping. Eur J Epidemiol 16, 447–453.[CrossRef]
    [Google Scholar]
  7. Esteve, C., Valera, L., Gutiérrez, C. & Ventosa, A. ( 2003; ). Taxonomic study of sucrose-positive Aeromonas jandaei-like isolates from faeces, water and eels: emendation of A. jandaei Carnahan et al. 1992. Int J Syst Evol Microbiol 53, 1411–1419.[CrossRef]
    [Google Scholar]
  8. Fukushima, M., Kakinuma, K. & Kawaguchi, R. ( 2002; ). Phylogenetic analysis of Salmonella, Shigella, and Escherichia coli strains on the basis of the gyrB gene sequence. J Clin Microbiol 40, 2779–2785.[CrossRef]
    [Google Scholar]
  9. Harf-Monteil, C., Le Flèche, A., Riegel, P., Prévost, G., Bermond, D., Grimont, P. A. D. & Monteil, H. ( 2004; ). Aeromonas simiae sp. nov., isolated from monkey faeces. Int J Syst Evol Microbiol 54, 481–485.[CrossRef]
    [Google Scholar]
  10. Huys, G., Coopman, R., Janssen, P. & Kersters, K. ( 1996; ). High-resolution genotypic analysis of the genus Aeromonas by AFLP fingerprinting. Int J Syst Bacteriol 46, 572–580.[CrossRef]
    [Google Scholar]
  11. Huys, G., Kämpfer, P., Altwegg, M., Coopman, R., Janssen, P., Gillis, M. & Kersters, K. ( 1997a; ). Inclusion of Aeromonas DNA hybridization group 11 in Aeromonas encheleia and extended descriptions of the species Aeromonas eucrenophila and A. encheleia. Int J Syst Bacteriol 47, 1157–1164.[CrossRef]
    [Google Scholar]
  12. Huys, G., Kämpfer, P., Altwegg, M. & 7 other authors ( 1997b; ). Aeromonas popoffii sp. nov., a mesophilic bacterium isolated from drinking water production plant reservoirs. Int J Syst Bacteriol 47, 1165–1171.[CrossRef]
    [Google Scholar]
  13. Huys, G., Kämpfer, P., Albert, M. J., Kühn, I., Denys, R. & Swings, J. ( 2002; ). Aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in Bangladesh, and extended description of Aeromonas hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980). Int J Syst Evol Microbiol 52, 705–712.[CrossRef]
    [Google Scholar]
  14. Huys, G., Pearson, M., Kämpfer, P., Denys, R., Cnockaert, M., Inglis, V. & Swings, J. ( 2003; ). Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand. Int J Syst Evol Microbiol 53, 885–891.[CrossRef]
    [Google Scholar]
  15. Huys, G., Cnockaert, M. & Swings, J. ( 2005; ). Aeromonas culicicola Pidiyar et al. 2002 is a later subjective synonym of Aeromonas veronii Hickman-Brenner et al. 1987. Syst Appl Microbiol 28, 604–609.[CrossRef]
    [Google Scholar]
  16. Joseph, S. W. & Carnahan, A. M. ( 2000; ). Update on the genus Aeromonas. ASM News 66, 218–223.
    [Google Scholar]
  17. Klenk, H.-P. & Zillig, W. ( 1994; ). DNA-dependent RNA polymerase subunit B as a tool for phylogenetic reconstructions: branching topology of the archeal domain. J Mol Evol 38, 420–432.[CrossRef]
    [Google Scholar]
  18. Korczak, B., Christensen, H., Emler, S., Frey, J. & Kuhnert, P. ( 2004; ). Phylogeny of the family Pasteurellaceae based on rpoB sequences. Int J Syst Evol Microbiol 54, 1393–1399.[CrossRef]
    [Google Scholar]
  19. Korczak, B. M., Stieber, R., Emler, S., Burnens, A. P., Frey, J. & Kuhnert, P. ( 2006; ). Genetic relatedness within the genus Campylobacter inferred from rpoB sequences. Int J Syst Evol Microbiol 56, 937–945.[CrossRef]
    [Google Scholar]
  20. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  21. Martin-Carnahan, A. & Joseph, S. W. ( 2005; ). Genus I. Aeromonas Stanier 1943, 213AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 557–578. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  22. Martínez-Murcia, A. J. ( 1999; ). Phylogenetic positions of Aeromonas encheleia, Aeromonas popoffii, Aeromonas DNA hybridization group 11 and Aeromonas group 501. Int J Syst Bacteriol 49, 1403–1408.[CrossRef]
    [Google Scholar]
  23. Martinez-Murcia, A. J., Benlloch, S. & Collins, M. D. ( 1992; ). Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridization. Int J Syst Bacteriol 42, 412–421.[CrossRef]
    [Google Scholar]
  24. Miñana-Galbis, D., Farfán, M., Fusté, M. C. & Lorén, J. G. ( 2004; ). Aeromonas molluscorum sp. nov., isolated from bivalve molluscs. Int J Syst Evol Microbiol 54, 2073–2078.[CrossRef]
    [Google Scholar]
  25. Mollet, C., Drancourt, M. & Raoult, D. ( 1997; ). rpoB sequence analysis as a novel basis for bacterial identification. Mol Microbiol 26, 1005–1011.[CrossRef]
    [Google Scholar]
  26. Morandi, A., Zhaxybayeva, O., Gogarten, J. P. & Gral, J. ( 2005; ). Evolutionary and diagnostic implications of intragenomic heterogeneity in the 16S rRNA gene in Aeromonas strains. J Bacteriol 187, 6561–6564.[CrossRef]
    [Google Scholar]
  27. Ovchinnikov, Y. A., Monastyrskaya, G. S., Gubanov, V. V. & 9 other authors ( 1981; ). The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the β-subunit. Eur J Biochem 116, 621–629.[CrossRef]
    [Google Scholar]
  28. Pavan, M. E., Abbott, S. L., Zorzopulos, J. & Janda, J. M. ( 2000; ). Aeromonas salmonicida subsp. pectinolytica subsp. nov., a new pectinase-positive subspecies isolated from a heavily polluted river. Int J Syst Evol Microbiol 50, 1119–1124.[CrossRef]
    [Google Scholar]
  29. Pidiyar, V., Kaznowski, A., Badri Narayan, N., Patole, M. & Shouche, Y. S. ( 2002; ). Aeromonas culicicola sp. nov., from the midgut of Culex quinquefasciatus. Int J Syst Evol Microbiol 52, 1723–1728.[CrossRef]
    [Google Scholar]
  30. Pidiyar, V. J., Jangid, K., Dayananda, K. M., Kaznowski, A., Gonzalez, J. M., Patole, M. S. & Shouche, Y. S. ( 2003; ). Phylogenetic affiliation of Aeromonas culicicola MTCC 3249T based on gyrB gene sequence and PCR-amplicon sequence analysis of cytolytic enterotoxin gene. Syst Appl Microbiol 26, 197–202.[CrossRef]
    [Google Scholar]
  31. Radice, F., Orlandi, V., Massa, V., Cavalca, L., Demarta, A., Wood, T. K. & Barbieri, P. ( 2006; ). Genotypic characterization and phylogenetic relations of Pseudomonas sp. (formerly P. stutzeri) OX1. Curr Microbiol 52, 395–399.[CrossRef]
    [Google Scholar]
  32. Sneath, P. H. A. ( 1993; ). Evidence from Aeromonas for genetic crossing-over in ribosomal sequences. Int J Syst Bacteriol 43, 626–629.[CrossRef]
    [Google Scholar]
  33. Soler, L., Yáñez, M. A., Chacon, M. R., Aguilera-Arreola, M. G., Catalán, V., Figueras, M. J. & Martínez-Murcia, A. J. ( 2004; ). Phylogenetic analysis of the genus Aeromonas based on two housekeeping genes. Int J Syst Evol Microbiol 54, 1511–1519.[CrossRef]
    [Google Scholar]
  34. Stackebrandt, E. & Goebel, B. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  35. Stackebrandt, E., Frederiksen, W., Garrity, G. M. & 10 other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  36. Urwin, R. & Maiden, M. C. ( 2003; ). Multi-locus sequence typing: a tool for global epidemiology. Trends Microbiol 11, 479–487.[CrossRef]
    [Google Scholar]
  37. Woese, C. R., Kandler, O. & Wheelis, M. L. ( 1990; ). Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eukarya. Proc Natl Acad Sci U S A 87, 4576–4579.[CrossRef]
    [Google Scholar]
  38. Yamamoto, S. & Harayama, S. ( 1995; ). PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61, 1104–1109.
    [Google Scholar]
  39. Yamamoto, S. & Harayama, S. ( 1996; ). Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products. Int J Syst Bacteriol 46, 506–511.[CrossRef]
    [Google Scholar]
  40. Yamamoto, S., Bouvet, P. J. M. & Harayama, S. ( 1999; ). Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA–DNA hybridization. Int J Syst Bacteriol 49, 87–95.[CrossRef]
    [Google Scholar]
  41. Yáñez, M. A., Catalán, V., Apráiz, D., Figueras, M. J. & Martínez-Murcia, A. J. ( 2003; ). Phylogenetic analysis of members of the genus Aeromonas based on gyrB gene sequences. Int J Syst Evol Microbiol 53, 875–883.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63650-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63650-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error