1887

Abstract

A Gram-negative bacterium, designated LA31B, was isolated from water collected from a hypersaline lagoon on Laysan Atoll in the north-western Hawaiian Islands. Single cells of LA31B were slightly curved but became helical as their length increased. Preliminary characterization based on 16S rRNA gene sequence analysis showed that LA31B shared 96·0 % identity with an sp. isolated from a cyanobacterial mat in hypersaline Lake Sinai, and 94 % identity with , the type species of the genus . A polyphasic taxonomic study was conducted and confirmed the phylogenetic affiliation of strain LA31B to the genus . However, LA31B was found to be distinct from all recognized species, by a comprehensive biochemical test analysis, whole-cell fatty acid profiling, DNA G+C content (35 mol% in LA31B) and degree of DNA–DNA reassociation. Most notably, LA31B was found to be an obligate halophile, a hitherto undescribed feature among recognized species. These data indicate that LA31B should be considered to represent a novel species in the genus , for which the name sp. nov. is proposed. This is the first obligately halophilic member of the genus. The type strain is LA31B (=ATCC BAA-1022=CIP 108450).

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2005-05-01
2019-12-13
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