1887

Abstract

Two bacterial isolates from the sputa of a patient with a pulmonary infection were subjected to a polyphasic taxonomic study. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and mycolic acids consistent with the profile for the genus . Comparative 16S rRNA gene sequencing showed that these isolates constitute a distinct subline within the genus , displaying 99·6–95·5 % sequence similarities with established species. However, DNA–DNA hybridization studies demonstrated unambiguously that the isolates are genealogically distinct from closely related species, namely and , which show high levels of 16S rRNA sequence similarity (99·2 and 99·6 % sequence similarity, respectively). On the basis of both phenotypic and phylogenetic evidence, it is proposed that these isolates be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMMIB N-402 (=CCUG 50200=CIP 108553).

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2005-07-01
2020-01-29
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