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Abstract

To obtain porcine isolates related to , we screened strains from piglet intestine grown on -specific MRS agar for hybridization to a fluorescent 16S rRNA-targeted DNA probe. Six strains were isolated and further characterized by phenotypic and molecular taxonomic methods. The isolates were Gram-positive, catalase-negative, facultatively anaerobic rods. They had similar phenotypic characteristics and displayed genomic DNA–DNA relatedness values of >78 % to each other, indicating that they belong to a single species. Comparative 16S rRNA gene sequence analysis demonstrated that the novel isolates were members of rRNA group I, which includes , the type species of the genus. Based on 16S rRNA gene sequence similarity, (99 %), (98 %) and (97 %) were the nearest relatives of the novel isolates, but their DNA–DNA relatedness was found to be lower than 49 %. One of the isolates, strain OTU171-001, was further characterized using physiological and biochemical tests. Together, the results enabled genotypic and phenotypic differentiation of strain OTU171-001 from the other species that showed 16S rRNA gene sequence similarity values greater than 97 %. Strain OTU171-001 merits species status and the name sp. nov. is proposed. The type strain is OTU171-001 (=DSM 16698=NCCB 100067).

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2006-01-01
2019-10-21
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