1887

Abstract

A Gram-negative, non-motile, non-spore-forming, rod-shaped bacterium (strain Y9) was isolated from a contaminated culture of the phototrophic bacterium , and was investigated using a polyphasic taxonomic approach. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y9 belonged to the order in the . Comparison of phylogenetic data indicated that it was most closely related to (98.5 % similarity of 16S rRNA gene sequence), and the phylogenetic distance from any other species of the order with a validly published name was greater than 7.5 % (i.e. less than 92.5 % similarity). The predominant ubiquinone was Q-10 and the major fatty acids were C, C, C cyclo 8 and C. The G+C content of genomic DNA of strain Y9 was 65.1 mol%. The results of DNA–DNA hybridization in combination with chemotaxonomic and physiological data demonstrated that strain Y9 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is Y9 (=KCTC 12246=NBRC 100803)

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2006-07-01
2021-03-03
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References

  1. Buck J. D. 1982; Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993
    [Google Scholar]
  2. Cappuccino J. G., Sherman N. 2002 Microbiology: a Laboratory Manual , 6th edn. Menlo Park, CA: Benjamin Cummings Science Publishing;
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Fitch W. M. 1972; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
    [Google Scholar]
  6. Garrity G. M., Holt J. G. 2001; The road map to the Manual . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  119–166 Edited by Garrity G. M., Boone D. R., Castenholz R. W. New York: Springer;
    [Google Scholar]
  7. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  8. Kim M. K., Im W.-T., Ohta H., Lee M., Lee S.-T. 2005; Sphingopyxis granuli sp. nov., a β -glucosidase producing bacterium in the family Sphingomonadaceae in α -4 subclass of the Proteobacteria . J Microbiol 43:152–157
    [Google Scholar]
  9. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Poly F., Monrozier L. J., Bally R. 2001; Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol 152:95–103 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI;
    [Google Scholar]
  15. Shin Y. K., Lee J.-S., Chun C. O., Kim H.-J., Park Y.-H. 1996; Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T . J Microbiol Biotechnol 6:68–69
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  19. Xie C.-H., Yokota A. 2005; Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa . Int J Syst Evol Microbiol 55:1223–1237 [CrossRef]
    [Google Scholar]
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