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Abstract

A psychrotolerant, Gram-negative, motile bacterium, designated CK 47, was isolated from sea water off the subantarctic Kerguelen islands (50° 40′ S 68° 25′ E). The isolate grew optimally at 22 °C and minimum and maximum temperature of growth were 4 and 37 °C, respectively. It required Na for growth and exhibited optimum growth at pH 8·5 and 4 % NaCl. It utilized hexane, heptane and petroleum ether as sole sources of carbon. Strain CK 47 had Q9 as the major respiratory quinone and C (21·7 %), C (21·3 %), C (5·7 %), C 7 (9·0 %) and C 9 (31·4 %) as predominant fatty acids. The G+C content of the DNA was 58 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that CK 47 formed a coherent cluster within the genus . It exhibited highest 16S rRNA gene sequence similarity of 96·8 % with . However, the level of DNA–DNA relatedness between strain CK47 and was only 55 %. On the basis of phenotypic characteristics, and phylogenetic and genotypic distinctiveness, strain CK 47 is considered to represent a novel species of the genus . The name sp. nov. is proposed, with CK 47 (=JCM 12521=MTCC 6519) as the type strain.

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2005-07-01
2020-01-25
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vol. , part 4, pp. 1453 - 1456

Phenotypic characteristics that differentiate sp. nov. CK47 from other species.

Comparison of the cellular fatty acid compositions of CK 47 and DSM 15157

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