1887

Abstract

A novel mesophilic, chemolithoautotrophic, sulfur-oxidizing bacterium, designated strain SO07, was isolated from a microaerobic waste-water biofilm. Chemolithoautotrophic growth was observed with elemental sulfur, sulfide and thiosulfate as sole electron donors and oxygen as electron acceptor. Anaerobic and heterotrophic growth were not observed. Nitrate was not used as a terminal electron acceptor. The optimum pH and temperature for growth were pH 7·5 and 30 °C, respectively. The major isoprenoid quinone was Q-8. The DNA G+C content of strain SO07 was 47·1 mol%. Phylogenetic analysis of 16S rRNA gene sequences demonstrated that strain SO07 formed a monophyletic group in the - with only 89 % similarity to members of the genus , its nearest phylogenetic neighbours. In addition, the isolate differed from members of the genus in its requirement for and tolerance of NaCl; strain SO07 was unable to grow in NaCl concentrations of more than 180 mM. On the basis of phylogenetic, chemotaxonomic and physiological data, it is proposed that isolate SO07 (=JCM 12417=ATCC BAA-1033) represents the type strain of a novel species in a new genus, gen. nov., sp. nov.

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2005-05-01
2020-01-22
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