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Abstract

During a study in the Argentinian region of Chaco (Córdoba), some strains were isolated from the rhizosphere of grasses growing in semi-desertic arid soils. Two of these strains, one isolated from the rhizospheric soil of (strain CH01) and the other from (strain PA01), were Gram-negative, strictly aerobic rods, which formed yellow round colonies on nutrient agar. They produced a water-insoluble yellow pigment, and a fluorescent pigment was also detected. A polyphasic taxonomic approach was used to characterize the strains. Comparison of the 16S rRNA gene sequences showed a similarity of 99·3 % between them, and phylogenetic analysis revealed that the strains belong to the genus , within the -subclass of the . The closest related species is IAM 1598 (similarity of 99·0 % to strain CH01 and 98·8 % to strain PA01), clustering in a separate branch with the various methods of tree building used. Strains CH01 and PA01 both had a single polar flagellum, like other yellow pigment-producing pseudomonads related to them. Both strains produced catalase and oxidase. Similar to , they did not hydrolyse gelatin or casein. The G+C DNA contents determined were 57·5 mol% for CH01 and 58·0 mol% for PA01. DNA–DNA hybridization results showed 81 % relatedness between them, and only 40–44 % relatedness with respect to the type strain of . These results, together with other phenotypic characteristics, support the conclusion that both isolates belong to the same species, and should be described as representing a novel species within the genus , for which the name sp. nov. is proposed. The type strain is CH01 (=LMG 22563=CECT 7010).

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2005-05-01
2020-01-17
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rep-PCR patterns for strains of and sp. nov. [PDF](21 KB)

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Neighbour-joining tree for sp. nov. and related species of the genus [PDF](16 KB)

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Similarity matrix data and reciprocal values for percentage DNA-DNA hybridization for strains included in this study. [PDF](17 KB)

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