1887

Abstract

Two bacterial strains, B-5 and NO1A, were isolated from the surface water of the Bohai Sea and deep-sea sediment of the east Pacific Ocean, respectively. Both strains were halophilic, aerobic, Gram-negative, non-spore-forming, catalase- and oxidase-positive motile rods. They grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. On the basis of 16S rRNA gene sequence similarity, strains B-5 and NO1A were shown to belong to the -. Highest similarity values were found with (95·2 %), (94·6 %) and (94·1 %). Principal fatty acids of both strains were C, C 7 and C 7. The chemotaxonomically characteristic fatty acid C cyclo 8 was also detected. On the basis of the above, together with results of physiological and biochemical tests, DNA–DNA hybridization, comparisons of 16S–23S internal transcribed spacer sequences and comparisons of the partial deduced amino acid sequence of alkane hydroxylase, both strains were affiliated to the genus but were differentiated from recognized species. Therefore, a novel species, sp. nov., represented by strains B-5 and NO1A is proposed, with the type strain B-5 (=DSM 16502=CGMCC 1.3690).

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2005-05-01
2019-09-20
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vol. , part 3, pp. 1181–1186

Transmission electron micrographs of cells of strains B-5 and NO1A and dendrograms showing the phylogenetic positions of the two strains plus recognized members of the genus based on 16S rRNA, ITS and gene sequences are available to download, together with a table giving DNA–DNA relatedness values. [PDF](455KB)



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