1887

Abstract

Three lactic acid bacterial (LAB) strains obtained from a Malaysian acid-fermented condiment, tempoyak (made from pulp of the durian fruit), showed analogous but distinct patterns after screening by SDS-PAGE of whole-cell proteins and comparison with profiles of all recognized LAB species. 16S rRNA gene sequencing of one representative strain showed that the taxon belongs phylogenetically to the genus , with its nearest neighbour being (98 % sequence similarity). Biochemical characteristics and DNA–DNA hybridization experiments demonstrated that the strains differ from and represent a single, novel species for which the name sp. nov. is proposed. The type strain is LMG 22556 (=LAB 1679=D-24=CCUG 49949).

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2005-05-01
2024-10-03
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References

  1. Antunes A., Rainey F. A., Nobre M. F., Schumann P., Ferreira A. M., Ramos A., Santos H., da Costa M. S. 2002; Leuconostoc ficulneum sp. nov., a novel lactic acid bacterium isolated from a ripe fig, and reclassification of Lactobacillus fructosus as Leuconostoc fructosum comb. nov. Int J Syst Evol Microbiol 52:647–655
    [Google Scholar]
  2. Carl G. D., Leisner J. J., Swings J., Vancanneyt M. 2004; Intra-specific variation of Lactobacillus plantarum and Lactobacillus pentosus in sensitivity towards various bacteriocins. Antonie van Leeuwenhoek 85:209–216 [CrossRef]
    [Google Scholar]
  3. Coenye T., Falsen E., Vancanneyt M., Hoste B., Govan J. R. W., Kersters K., Vandamme P. 1999; Classification of Alcaligenes faecalis -like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49:405–413 [CrossRef]
    [Google Scholar]
  4. De Vuyst L., Schrijvers V., Paramithiotis S., Hoste B., Vancanneyt M., Swings J., Kalantzopoulos G., Tsakalidou E., Messens W. 2002; The biodiversity of lactic acid bacteria in Greek traditional wheat sourdoughs is reflected in both composition and metabolite formation. Appl Environ Microbiol 68:6059–6069 [CrossRef]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. Gevers D., Huys G., Swings J. 2001; Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205:31–36 [CrossRef]
    [Google Scholar]
  7. Goris J., Suzuki K., de Vos P., Nakase T., Kersters K. 1998; Evaluation of a microplate DNA–DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44:1148–1153 [CrossRef]
    [Google Scholar]
  8. Gregersen T. 1978; Rapid method for distinction of Gram negative from Gram positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [CrossRef]
    [Google Scholar]
  9. Leisner J. J., Millan J. C., Huss H. H., Larsen L. M. 1994; Production of histamine and tyramine by lactic acid bacteria isolated from vacuum-packed sugar-salted fish. J Appl Bacteriol 76:417–423 [CrossRef]
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Niemann S., Pühler A., Tichy H.-V., Simon R., Selbitschka W. 1997; Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population. J Appl Microbiol 82:477–484 [CrossRef]
    [Google Scholar]
  13. Pot B., Janssens D. 1993; The potential role of a culture collection for identification and maintenance of lactic acid bacteria. In The Lactic Acid Bacteria, Proceedings of the First Lactic Acid Computer Conference pp  81–87 Wymondham, UK: Horizon Scientific Press;
    [Google Scholar]
  14. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  15. Shaw B. G., Harding C. D. 1984; A numerical taxonomic study of lactic acid bacteria from vacuum-packed beef, pork, lamb and bacon. J Appl Bacteriol 56:25–40 [CrossRef]
    [Google Scholar]
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