1887

Abstract

Recent studies on the occurrence of (per)chlorate-reducing bacteria have resulted in the characterization of strains capable of dissimilatory (per)chlorate reduction. Phylogenetic analysis has shown that these bacteria are members of the . Strains have been isolated from polluted and pristine sites, but only strains from polluted sites have been characterized in detail and deposited in culture collections. Herein we describe the isolation and characterization of perchlorate-reducing bacterium strain MA-1 and chlorate-reducing bacterium strain ASK-1, respectively isolated from a pristine and a chlorate-polluted site. Both isolates are members of the . The 16S rRNA gene sequence similarity of MA-1 to DSM 13637 is 97·6 %, but the relatedness in DNA–DNA reassociation is only 37 %. Therefore, we propose to classify strain MA-1 (=DSM 15637=ATCC BAA-776) as the type strain of a novel species, sp. nov. Strain ASK-1 and a previously described strain GR-1 show 100 and 99 % 16S rRNA gene sequence similarity to DSM 13592 and DSM 13638, respectively. DNA–DNA hybridization studies indicated that strains ASK-1 and GR-1 are related at the species level to DSM 13592 (79 %) and DSM 13638 (85 %), respectively. As suggested previously, appears to be a later heterotypic synonym of . Although strain ASK-1 is identified as , its morphology and growth requirements are different from those of the type strain.

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2005-09-01
2019-10-23
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(A, B) Colonies of (A) and strain ASK-1 (B) grown on nutrient agar medium with oxygen as electron acceptor. (C, D) Cell extracts of (C) and strain ASK-1 (D) from batch cultures, both grown with chlorate and acetate as electron acceptor and electron donor, respectively.

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Transmission electron micrograph of cells of strain GR-1.

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