1887

Abstract

Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of . strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that -glutamic acid, although not essential for growth, was utilized by both species. The species possessed an uptake system specific for -glutamic acid, whereas the species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K antiport system and similar energy systems for the uptake of -glutamic acid. The rate of uptake by the species type strain was 20-fold greater than that shown by the species type strain. The names sp. nov. and sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657 (=LMG 22481=CIP 108307) and Logan B-1599 (LMG 22483=CIP 108308), respectively.

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2005-05-01
2019-12-14
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vol. , part 3, pp. 1039–1050

Graphs showing the effects of ionophores on glutamate uptake (Fig. A) and tables giving the fatty acid profiles (Table A) and the effects of various competitors (Table B) and energy inhibitors (Table C) on glutamate uptake in strains LMG 22481 and LMG 22483 are available to download. [PDF](121KB)



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