1887

Abstract

An actinomycete, strain B60, was isolated from a soil sample in Napier, Western Cape province of South Africa. Based on 16S rRNA gene sequence analysis and chemotaxonomy, strain B60 was identified as a member of the genus . Strain B60 produced an antibiotic with activity against , and , but not against . Significant differences in morphological and physiological characteristics indicate that strain B60 represents a novel species of the genus . The name sp. nov. is proposed. The type strain is B60 (=DSM 44846=NRRL B-24319).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63359-0
2005-03-01
2021-04-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550703.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63359-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  2. Betina V. 1973; Bioautography in paper and thin-layer chromatography and its scope in the antibiotic field. J Chromatogr 78:41–51 [CrossRef]
    [Google Scholar]
  3. Chou S., Chedore P., Kasatiya S. 1998; Use of gas chromatographic fatty acid and mycolic acid cleavage product determination to differentiate among Mycobacterium genavense , Mycobacterium fortuitum , Mycobacterium simiae , and Mycobacterium tuberculosis . J Clin Microbiol 36:577–579
    [Google Scholar]
  4. Cook A. E., Meyers P. R. 2003; Rapid identification of filamentous actinomycetes to the genus level using genus-specific 16S rRNA gene restriction fragment patterns. Int J Syst Evol Microbiol 53:1907–1915 [CrossRef]
    [Google Scholar]
  5. Goodfellow M. 1989; Maduromycetes. In Bergey's Manual of Systematic Bacteriology vol 4 pp  2509–2510 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  6. Hacène H., Lefebvre G. 1995; AH17, a new non-polyenic antifungal antibiotic produced by a strain of Spirillospora . Microbios 83:199–205
    [Google Scholar]
  7. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  8. Keswani J., Whitman W. B. 2001; Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotes. Int J Syst Evol Microbiol 51:667–678
    [Google Scholar]
  9. Komagata K., Suzuki K. L. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  10. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A. 1984; Saccharothrix : a new genus of the Actinomycetales related to Nocardiopsis . Int J Syst Bacteriol 34:426–431 [CrossRef]
    [Google Scholar]
  11. Lechevalier M. P., de Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Ecol Syst 5:249–260 [CrossRef]
    [Google Scholar]
  12. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance–temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 7:195–206
    [Google Scholar]
  13. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium , Nocardia , and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204 [CrossRef]
    [Google Scholar]
  14. Quintana E. T., Trujillo M. E., Goodfellow M. 2003; Actinomadura mexicana sp. nov. and Actinomadura meyerii sp. nov., two novel soil sporoactinomycetes. Syst Appl Microbiol 26:511–517 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989; Bacterial media, antibiotics and bacterial strains. In Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  19. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  20. Trujillo M. E., Goodfellow M. 1997; Polyphasic taxonomic study of clinically significant actinomadurae including the description of Actinomadura latina sp. nov. Zentbl Bakteriol 285:212–233 [CrossRef]
    [Google Scholar]
  21. Weyer K., Groenewald P., Zwarenstein M., Lombard C. J. 1995; Tuberculosis drug resistance in the Western Cape. S Afr Med J 86:499–504
    [Google Scholar]
  22. WHO 2000; Tuberculosis . Fact sheet no. 104. Geneva: World Health Organization; http://www.who.int/mediacentre/factsheets/fs104/en/
  23. WHO 2004 Discussion. In Global Tuberculosis Control , WHO Report 2004 pp  44–47 Geneva: World Health Organization; http://www.who.int/tb/publications/global_report/2004/en/discussion.pdf
    [Google Scholar]
  24. Zhang Z., Wang Y., Ruan J. 1998; Reclassification of Thermomonospora and Microtetraspora . Int J Syst Bacteriol 48:411–422 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63359-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63359-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error